EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | alpha-D-glucose-methylenephosphonate | competitive inhibitor | Thermus thermophilus | |
2.4.1.1 | gluconolactone | competitive inhibitor | Thermus thermophilus | |
2.4.1.1 | NaAsO2 | competitive inhibitor with respect to phosphate | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 1.2 | - |
alpha-D-glucose 1-phosphate | synthesis reaction, cosubstrate: maltotetraose, pH 6.5, 70°C | Thermus thermophilus | |
2.4.1.1 | 1.7 | - |
maltoheptaose | degradation direction, cosubstrate: phosphate, pH 6.5, 30°C | Thermus thermophilus | |
2.4.1.1 | 1.9 | - |
phosphate | degradation direction, cosubstrate: maltoheptaose, pH 6.5, 30°C | Thermus thermophilus | |
2.4.1.1 | 2.2 | - |
phosphate | degradation direction, cosubstrate: maltoheptaose, pH 6.5, 70°C | Thermus thermophilus | |
2.4.1.1 | 2.8 | - |
maltotetraose | degradation direction, cosubstrate: phosphate, pH 6.5, 30°C | Thermus thermophilus | |
2.4.1.1 | 3.6 | - |
maltoheptaose | degradation direction, cosubstrate: phosphate, pH 6.5, 70°C | Thermus thermophilus | |
2.4.1.1 | 4.8 | - |
maltotetraose | degradation direction, cosubstrate: phosphate, pH 6.5, 70°C | Thermus thermophilus | |
2.4.1.1 | 12.7 | - |
maltotetraose | synthesis reaction, cosubstrate: alpha-D-glucose 1-phosphate, pH 6.5, 70°C | Thermus thermophilus | |
2.4.1.1 | 13.4 | - |
maltotriose | synthesis reaction, cosubstrate: alpha-D-glucose 1-phosphate, pH 6.5, 70°C | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Thermus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | - |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | glycogen + phosphate | - |
Thermus thermophilus | alpha-D-glucose 1-phosphate + ? | - |
? | |
2.4.1.1 | maltoheptaose + phosphate | - |
Thermus thermophilus | maltohexaose + alpha-D-glucose 1-phosphate | - |
? | |
2.4.1.1 | maltotetraose + phosphate | - |
Thermus thermophilus | maltotriose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | maltotriose + alpha-D-glucose 1-phosphate | - |
Thermus thermophilus | maltotetraose + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | dimer | 2 * 90000, While at 25°C the subunit composition of the thermophilic enzyme is an octameric form, the preferential form at the optimum temperature of 70°C seems to be a dimer | Thermus thermophilus |
2.4.1.1 | octamer | 8 * 90000, While at 25°C the subunit composition of the thermophilic enzyme is an octameric form, the preferential form at the optimum temperature of 70°C seems to be a dimer | Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 70 | - |
in the pH range 5.5-6.5 | Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 50 | 90 | 50°C: about 50% of maximal activity, 90°C: about 40% of maximal activity | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | pyridoxal 5'-phosphate | the enzyme contains 1 M pyridoxal 5'-phosphate per M of subunit | Thermus thermophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 0.1 | - |
alpha-D-glucose-methylenephosphonate | 70°C, pH not specified in the publication | Thermus thermophilus | |
2.4.1.1 | 3.3 | - |
gluconolactone | 70°C, pH not specified in the publication | Thermus thermophilus | |
2.4.1.1 | 4.1 | - |
NaAsO2 | 70°C, pH not specified in the publication | Thermus thermophilus |