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Literature summary extracted from

  • Nguyen, M.; Quemard, A.; Marrakchi, H.; Bernadou, J.; Meunier, B.
    The nonenzymatic activation of isoniazid by MnIII-pyrophosphate in the presence of NADH produces the inhibition of the enoyl-ACP reductase InhA from Mycobacterium tuberculosis (2001), Chemistry, 4, 35-40 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 overexpression in Escherichia coli Mycobacterium tuberculosis
1.3.1.118 overexpression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 isoniazid fast and efficient inhibition of InhA in the presence of NADH and INH using MnIII-pyrophosphate as nonenzymatic reagent. This chemical oxidant might be a useful tool for further mechanistic studies of isoniazid activation in attempts to establish the exact structures of isoniazid reactive species and InhA inhibitor complex(es). Mycobacterium tuberculosis
1.3.1.118 isoniazid fast and efficient inhibition of InhA in the presence of NADH and INH using MnIII-pyrophosphate as nonenzymatic reagent. This chemical oxidant might be a useful tool for further mechanistic studies of isoniazid activation in attempts to establish the exact structures of isoniazid reactive species and InhA inhibitor complex(es). Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Mycobacterium tuberculosis
-
-
-
1.3.1.118 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9
-
 Mycobacterium tuberculosis
1.3.1.118
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 trans-2-decenoyl-CoA + NADH + H+
-
 Mycobacterium tuberculosis decanoyl-CoA + NAD+
-
 ?
1.3.1.118 trans-2-decenoyl-CoA + NADH + H+
-
 Mycobacterium tuberculosis decanoyl-CoA + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 InhA
-
 Mycobacterium tuberculosis
1.3.1.118 InhA
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.9 25
-
 assay at Mycobacterium tuberculosis
1.3.1.118 25
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 7.5
-
 assay at Mycobacterium tuberculosis
1.3.1.118 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Mycobacterium tuberculosis
1.3.1.118 NADH
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.3.1.9 metabolism the enzyme is involved on the biosynthetic pathway for mycolic acids Mycobacterium tuberculosis
1.3.1.118 metabolism the enzyme is involved on the biosynthetic pathway for mycolic acids Mycobacterium tuberculosis