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Literature summary extracted from

  • Ideno, N.; Umeyama, S.; Watanabe, T.; Nakajima, M.; Sato, T.; Hoshino, T.
    Alicyclobacillus acidocaldarius squalene-hopene cyclase the critical role of steric bulk at Ala306 and the first enzymatic synthesis of epoxydammarane from 2,3-oxidosqualene (2018), ChemBioChem, 19, 1873-1886 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.17 expressed in Escherichia coli Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.17 A306F the mutant produces apolypodatetraene, gamma-polypodatetraene and neopolypoda-5(6),13,17,21-teraene from squalene and polypoda-8(9),13,17,21-tetraen-3a-ol, polypoda-7(8),13,17,21-tetraen-3alpha-ol and polypoda-7(8),13,17,21-tetraen-3beta-ol from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 A306T the mutant produces (20R)-dammara-13(17),24-dien-3a-ol, (20R)-dammara-13(17),24-dien-3b-ol, (20R)-dammara-12(13),24-dien-3a-ol, (20R)-dammara-12(13),24-dien-3b-ol and (20R,24R)-17-epi-epoxydammarane from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 A306V the mutant produces apolypodatetraene, dammara-13(17)-24-diene, gamma-polypodatetraene, (17E)(13aH)-malabarica-14(27),17,21-triene, (20R)-dammara-12(13),24-diene, (20R)-eupha-7(8),24-diene, (20S)-tirucalla-7(8),24-diene, and 17-epi-dammara-20(21),24-diene from squalene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.17 squalene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hopan-22-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 P33247
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.17 (3R)-2,3-oxidosqualene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 3alpha-hydroxyhopene + 3alpha-hydroxyhopanol
-
 ?
5.4.99.17 (3S)-2,3-oxidosqualene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 3beta-hydroxyhopene + 3beta-hydroxyhopanol
-
 ?
5.4.99.17 squalene
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hopan-22-ol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.17 SHC
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 squalene-hopene cyclase
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446