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Literature summary extracted from

  • Asif, M.; Bhalla, T.C.
    Hydroxynitrile lyase of wild apricot (Prunus armeniaca L.) purification, characterization and application in synthesis of enantiopure mandelonitrile (2017), Catal. Lett., 146, 1118-1127 .
No PubMed abstract available

Application

EC Number Application Comment Organism
4.1.2.10 agriculture the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals Prunus armeniaca
4.1.2.10 pharmacology the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals Prunus armeniaca
4.1.2.10 synthesis the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals Prunus armeniaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 Ca2+ 10 mM, 1% inhibition Prunus armeniaca
4.1.2.10 Cd2+ 10 mM, 3% inhibition Prunus armeniaca
4.1.2.10 Co2+ 10 mM, 22% inhibition Prunus armeniaca
4.1.2.10 Cs2+ 10 mM, 11% inhibition Prunus armeniaca
4.1.2.10 Cu2+ 10 mM, 21% inhibition Prunus armeniaca
4.1.2.10 Fe2+ 10 mM, 29% inhibition Prunus armeniaca
4.1.2.10 Fe3+ 10 mM, 22% inhibition Prunus armeniaca
4.1.2.10 Hg2+ 10 mM, complete inhibition; 10 mM, % inhibition Prunus armeniaca
4.1.2.10 Mg2+ 10 mM, 10% inhibition Prunus armeniaca
4.1.2.10 Mn2+ 10 mM, 17% inhibition Prunus armeniaca
4.1.2.10 Pb2+ 10 mM, 37% inhibition Prunus armeniaca
4.1.2.10 Sr2+ 10 mM, 15% inhibition Prunus armeniaca
4.1.2.10 W6+ 10 mM, 5% inhibition Prunus armeniaca
4.1.2.10 Zn2+ 10 mM, 2% inhibition Prunus armeniaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 3.76
-
 (R)-mandelonitrile pH 5.0, 25°C Prunus armeniaca
4.1.2.10 16.1
-
 benzaldehyde pH 5.0, 25°C Prunus armeniaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 360000
-
 gel filtration, native-PAGE Prunus armeniaca

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus armeniaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Prunus armeniaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 kernel
-
 Prunus armeniaca
-
4.1.2.10 seed
-
 Prunus armeniaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 141
-
 pH 5.0, 25°C Prunus armeniaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Prunus armeniaca cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + benzaldehyde synthesis of mandelonitrile is carried out with 89% molar conversion with 96% enantionmeric excess for R-mandelonitrile Prunus armeniaca (R)-mandelonitrile
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.10 ? x * 40000 + x * 37000, SDS-PAGE Prunus armeniaca

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 ParsHNL
-
 Prunus armeniaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 25
-
-
 Prunus armeniaca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.10 20 50 20°C: about 55% of maximal activity, 50°C: about 25% of maximal activity Prunus armeniaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 4
-
 the enzyme does not show any significant loss of activity at 4°C, hence it has good potential for industrial application Prunus armeniaca
4.1.2.10 25
-
 t1/2: 216 h Prunus armeniaca
4.1.2.10 30
-
 t1/2: 141 h Prunus armeniaca
4.1.2.10 50
-
 t1/2: 8.1 h Prunus armeniaca
4.1.2.10 60
-
 t1/2: 2.5 h Prunus armeniaca
4.1.2.10 70
-
 t1/2: 0.77 h Prunus armeniaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 43.3
-
 benzaldehyde pH 5.0, 25°C Prunus armeniaca
4.1.2.10 1130.4
-
 (R)-mandelonitrile pH 5.0, 25°C Prunus armeniaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 4.75 5 the enzyme shows maximum activity in 0.1 M sodium-citrate buffer having pH 4.75 at 25°C Prunus armeniaca

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.10 FAD FAD containing enzyme Prunus armeniaca

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.10 2.7
-
 benzaldehyde pH 5.0, 25°C Prunus armeniaca
4.1.2.10 300.6
-
 (R)-mandelonitrile pH 5.0, 25°C Prunus armeniaca