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Literature summary extracted from

  • Parisot, J.; Ghochikyan, A.; Langlois, V.; Sakanyan, V.; Rabiller, C.
    Exopolygalacturonate lyase from Thermotoga maritima Cloning, characterization and organic synthesis application (2002), Carbohydr. Res., 337, 1427-1433 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.22 synthesis the highly thermostable enzyme constitutes a useful catalyst for a simplified synthesis of (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid which is extremely difficult to obtain via chemical synthesis Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.22 overexpressed in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.22 EGTA
-
 Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.22 Ca2+ required Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.22 Thermotoga maritima Q9WYR4
-
-
4.2.2.22 Thermotoga maritima DSM 3109 Q9WYR4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.22 proteolytic modification precursor undergoes N-terminal processing by cleavage at a putative site between alanine and serine residues Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.22
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.22 polygalacturonic acid the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield Thermotoga maritima (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid
-
 ?
4.2.2.22 polygalacturonic acid the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield Thermotoga maritima DSM 3109 (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.22 ? x * 42000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
4.2.2.22 Pel
-
 Thermotoga maritima
4.2.2.22 Tm0433
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.22 100
-
 the enzyme loses 50% of its initial activity within 30 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.22 9.5
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.22 8.5 10 pH 8.5: about 70% of maximal activity, pH 10.0: about 50% of maximal activity. Below pH 7 or above pH 11, no appreciable activity can be detected Thermotoga maritima