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Literature summary extracted from
- Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose (2014), Can. J. Microbiol., 60, 599-604 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | recombinant expression in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | additional information | large-scale production of enzyme TreS in Escherichia coli for trehalose production, conversion of 59% maltose by the purified recombinant enzyme with about 5.1% D-glucose as by-product | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | Pseudomonas putida | - |
alpha,alpha-trehalose | - |
r |  |
| 5.4.99.16 | maltose | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Pseudomonas putida | Q88FN0 | - |
- |
| 5.4.99.16 | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | Q88FN0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | recombinant enzyme from Echerichia coli strain BL21(DE3) to 98% purity | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | - |
Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | conversion of 59% maltose by the purified recombinant enzyme with about 5.1% D-glucose as by-product | Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | - |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | conversion of 59% maltose by the purified recombinant enzyme with about 5.1% D-glucose as by-product | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | additional information | optimal activity of purified recombinant enzyme at 30% substrate concentration | Pseudomonas putida | ? | - |
? | |
| 5.4.99.16 | additional information | optimal activity of purified recombinant enzyme at 30% substrate concentration | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | ? | x * 67000, recombinant enzyme, SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | Trehalose synthase | - |
Pseudomonas putida |
| 5.4.99.16 | TreS | - |
Pseudomonas putida |
| 5.4.99.16 | treSB | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 55 | - |
recombinant enzyme | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 7.4 | - |
recombinant enzyme | Pseudomonas putida |
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