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Literature summary extracted from

  • Lee, E.; Jeong, B.C.; Park, Y.H.; Kim, H.H.
    Expression of the gene encoding oxalate decarboxylase from Bacillus subtilis and characterization of the recombinant enzyme (2014), BMC Res. Notes, 7, 598 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 expressed in Escherichia coli TOP 10 cells Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 Mn2+
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Bacillus subtilis
-
formate + CO2
-
?
4.1.1.2 oxalate + H+ Bacillus subtilis 128
-
formate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis O34714
-
-
4.1.1.2 Bacillus subtilis 128 O34714
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 Ni-NTA column chromatography, and Superdex 200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
Bacillus subtilis formate + CO2
-
?
4.1.1.2 oxalate + H+
-
Bacillus subtilis 128 formate + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 ? x * 43000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OXDC
-
Bacillus subtilis
4.1.1.2 pBy recombinant gene name Bacillus subtilis
4.1.1.2 YvrK
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.2 28
-
-
Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 5
-
-
Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.2 O2 purified oxalate decarboxylase degrades more than 50% of oxalate when incubated with MnCl2 and sodium oxalate in atmospheric O2 Bacillus subtilis