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Literature summary extracted from

  • Khosravi, C.; Kun, R.S.; Visser, J.; Aguilar-Pontes, M.V.; de Vries, R.P.; Battaglia, E.
    In vivo functional analysis of L-rhamnose metabolic pathway in Aspergillus niger a tool to identify the potential inducer of RhaR (2017), BMC Microbiol., 17, 214-227 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.90 gene LRA3, genetic organization, L-rhamnose catabolism genes RHA1, LRA2, LRA3 and LRA4 are organized in a chromosomal gene cluster in Scheffersomyces stipitis. In Scheffersomyces stipitis, another transcription factor than TRC1 is found in the L-rhamnose cluster, FST14. This transcription factor is more up-regulated than TRC1 on L-rhamnose and it might be co-regulating the pectinolytic genes together with TRC1 Scheffersomyces stipitis
4.2.1.90 gene lraC, genetic organization, genes lraA, lraB and lraC, as well as the L-rhamnose regulator RhaR are organized in a cluster Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.90 additional information construction of a DELTAlraC knockout mutant from strain CBS 141257, gene deletion and transcriptomic analysis is performed to elucidate the function of LraC, protoplast-mediated transformations of Aspergillus niger. Deletion of genes lraA, lraB or lraC causes a reduction in expression level of the other genes of the L-rhamnose pathway. Strongly reduced expression levels of lraA, lraB, rhaR, rglB, rgxA and rgaeA are observed in the DELTAlraC mutant upon the 2 h transfer to L-rhamnonate compared to the reference strain. But none of the deletions of genes lraA, lraB or lraC results in accumulation of the inducer of the pathway regulator (RhaR) Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.90 L-rhamnonate Aspergillus niger
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Scheffersomyces stipitis
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Aspergillus niger CBS 141257
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.90 Aspergillus niger
-
 the CBS 141257 strain is obtained by transformation of strain N593.20 with uridine (pyrG) from Aspergillus oryzae
-
4.2.1.90 Aspergillus niger CBS 141257
-
 the CBS 141257 strain is obtained by transformation of strain N593.20 with uridine (pyrG) from Aspergillus oryzae
-
4.2.1.90 Scheffersomyces stipitis A3LZU6 i.e. Pichia stipitis
-
4.2.1.90 Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545 A3LZU6 i.e. Pichia stipitis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.90 mycelium
-
 Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.90 L-rhamnonate
-
 Aspergillus niger 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Aspergillus niger CBS 141257 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.90 LRA3
-
 Scheffersomyces stipitis
4.2.1.90 LraC
-
 Aspergillus niger
4.2.1.90 PICST_50672
-
 Scheffersomyces stipitis

General Information

EC Number General Information Comment Organism
4.2.1.90 malfunction a double deletion of lraC and lraA in Aspergillus niger results in a strain that exhibits no L-rhamnose dehydrogenase activity and cannot consume or grow on L-rhamnose. Deletion of the clustered genes lraA, lraB or lraC causes a reduction in expression level of the other genes of the L-rhamnose pathway, rhtA is not expressed in the DELTAlraC and DELATrhaR mutants. Strongly reduced expression levels of lraA, lraB, rhaR, rglB, rgxA and rgaeA are observed in the DELTAlraC mutant upon the 2 h transfer to L-rhamnonate compared to the reference strain Aspergillus niger
4.2.1.90 metabolism L-rhamnonate dehydratase (LRA3) converts L-rhamnonate to 2-dehydro-3-deoxy-L-rhamnonate (L-KDR), catalyzing the third step of the non-phosphorylative pathway in Schefferomyces stipitis. The inducer of RhaR is beyond L-rhamnonate dehydratase (LraC) and is likely to be the 2-keto-3-L-deoxyrhamnonate Scheffersomyces stipitis
4.2.1.90 metabolism L-rhamnonate dehydratase (LraC) probably converts L-rhamnonate to 2-dehydro-3-deoxy-L-rhamnonate (L-KDR), catalyzing the third step of the non-phosphorylative pathway. The inducer of RhaR is beyond L-rhamnonate dehydratase (LraC) and is likely to be the 2-keto-3-L-deoxyrhamnonate Aspergillus niger