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Literature summary extracted from

  • Ke, D.; Caiyin, Q.; Zhao, F.; Liu, T.; Lu, W.
    Heterologous biosynthesis of triterpenoid ambrein in engineered Escherichia coli (2018), Biotechnol. Lett., 40, 399-404 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.B20 coexpression of onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module) under control of constitutive J23119 promoter, subcloning in Escherichia coli strain DH5alpha Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.B20 additional information establishing of an (+)-ambrein production system by coexpressing the onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module). The squalene synthase produces squalene, SHC enzyme mutant D377C produces 3-deoxyachilleol A from squalene, and BmTC forms ambrein from it, method optimization. Optimal temperature for ambrein production is 30-34°C Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.B20 3-deoxyachilleol A Priestia megaterium
-
(+)-ambrein
-
?
4.2.1.B20 8alpha-hydroxypolypoda-13,17,21-triene Priestia megaterium
-
onoceranoxide + 14beta-hydroxyonocera-8(26)-ene
-
?
4.2.1.B20 squalene Priestia megaterium
-
8alpha-hydroxypolypoda-13,17,21-triene
-
?
4.2.1.B20 tetraprenyl-beta-curcumene + H2O Priestia megaterium
-
baciterpenol A
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.B20 Priestia megaterium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.B20 3-deoxyachilleol A
-
Priestia megaterium (+)-ambrein
-
?
4.2.1.B20 8alpha-hydroxypolypoda-13,17,21-triene
-
Priestia megaterium onoceranoxide + 14beta-hydroxyonocera-8(26)-ene
-
?
4.2.1.B20 squalene
-
Priestia megaterium 8alpha-hydroxypolypoda-13,17,21-triene
-
?
4.2.1.B20 tetraprenyl-beta-curcumene + H2O
-
Priestia megaterium baciterpenol A
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.B20 BmeTC
-
Priestia megaterium
4.2.1.B20 tetraprenyl-beta-curcumene cyclase
-
Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.B20 30
-
in vivo assay at Priestia megaterium