EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.B20 | coexpression of onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module) under control of constitutive J23119 promoter, subcloning in Escherichia coli strain DH5alpha | Priestia megaterium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.B20 | additional information | establishing of an (+)-ambrein production system by coexpressing the onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module). The squalene synthase produces squalene, SHC enzyme mutant D377C produces 3-deoxyachilleol A from squalene, and BmTC forms ambrein from it, method optimization. Optimal temperature for ambrein production is 30-34°C | Priestia megaterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.B20 | 3-deoxyachilleol A | Priestia megaterium | - |
(+)-ambrein | - |
? | |
4.2.1.B20 | 8alpha-hydroxypolypoda-13,17,21-triene | Priestia megaterium | - |
onoceranoxide + 14beta-hydroxyonocera-8(26)-ene | - |
? | |
4.2.1.B20 | squalene | Priestia megaterium | - |
8alpha-hydroxypolypoda-13,17,21-triene | - |
? | |
4.2.1.B20 | tetraprenyl-beta-curcumene + H2O | Priestia megaterium | - |
baciterpenol A | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.B20 | Priestia megaterium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.B20 | 3-deoxyachilleol A | - |
Priestia megaterium | (+)-ambrein | - |
? | |
4.2.1.B20 | 8alpha-hydroxypolypoda-13,17,21-triene | - |
Priestia megaterium | onoceranoxide + 14beta-hydroxyonocera-8(26)-ene | - |
? | |
4.2.1.B20 | squalene | - |
Priestia megaterium | 8alpha-hydroxypolypoda-13,17,21-triene | - |
? | |
4.2.1.B20 | tetraprenyl-beta-curcumene + H2O | - |
Priestia megaterium | baciterpenol A | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.B20 | BmeTC | - |
Priestia megaterium |
4.2.1.B20 | tetraprenyl-beta-curcumene cyclase | - |
Priestia megaterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.B20 | 30 | - |
in vivo assay at | Priestia megaterium |