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Literature summary extracted from
- Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima (2013), Biotechnol. Bioprocess Eng., 18, 897-902 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | synthesis | the enzyme could be useful for producing a low sulfated agar and electrophoretic grade agarose | Thermotoga maritima |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.6.1 | expressed in Escherichia coli W3110 with pCol-MICT as the vector | Thermotoga maritima |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 65000 | - |
SDS-PAGE | Thermotoga maritima |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.1 | Thermotoga maritima | Q9X229 | - |
- |
| 3.1.6.1 | Thermotoga maritima DSM 3109 | Q9X229 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.1 | partially purified | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.1 | 4-nitrophenyl sulfate + H2O | - |
Thermotoga maritima | 4-nitrophenol + sulfate | - |
? |  |
| 3.1.6.1 | 4-nitrophenyl sulfate + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + sulfate | - |
? | |
| 3.1.6.1 | agar + H2O | sulfate content of agar is diminished to 40% after a 12 h treatment | Thermotoga maritima | ? | - |
? | |
| 3.1.6.1 | agar + H2O | sulfate content of agar is diminished to 40% after a 12 h treatment | Thermotoga maritima DSM 3109 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | ? | x * 65000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 80 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 60 | 90 | 60°C: about 60% of maximal activity, 90°C: about 30% of maximal activity | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 7 | - |
- |
Thermotoga maritima |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 6 | 8 | pH 6.0: about 25% of maximal activity, pH 8.0: about 85% of maximal activity | Thermotoga maritima |
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Release 2023.1 (January 2023)
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