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Literature summary extracted from

  • Cheng, Z.; Peplowski, L.; Cui, W.; Xia, Y.; Liu, Z.; Zhang, J.; Kobayashi, M.; Zhou, Z.
    Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac-mandelonitrile by semi-rational engineering (2018), Biotechnol. Bioeng., 115, 524-535 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 expression in Escherichia coli Rhodococcus rhodochrous

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.84 A51L wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 74.3% Rhodococcus rhodochrous
4.2.1.84 F37H wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 96.8% Rhodococcus rhodochrous
4.2.1.84 F37H/F51L wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 94.6% Rhodococcus rhodochrous
4.2.1.84 F37H/L48A wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 95.1% Rhodococcus rhodochrous
4.2.1.84 F37Y wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 88.7% Rhodococcus rhodochrous
4.2.1.84 F51Q wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 60.2% Rhodococcus rhodochrous
4.2.1.84 L48Q wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 59.7% Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 38.2
-
 (S)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous
4.2.1.84 41.9
-
 (S)-mandelonitrile pH 7.4, 10°C, mutant enzyme F37H Rhodococcus rhodochrous
4.2.1.84 43.8
-
 (R)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.84 (R)-mandelonitrile + H2O Rhodococcus rhodochrous wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% (R)-mandelamide
-
 ?
4.2.1.84 (R)-mandelonitrile + H2O Rhodococcus rhodochrous J1 wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% (R)-mandelamide
-
 ?
4.2.1.84 (S)-mandelonitrile + H2O Rhodococcus rhodochrous wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% (S)-mandelamide
-
 ?
4.2.1.84 (S)-mandelonitrile + H2O Rhodococcus rhodochrous J1 wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% (S)-mandelamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus rhodochrous P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-
4.2.1.84 Rhodococcus rhodochrous J1 P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 (R)-mandelonitrile + H2O wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% Rhodococcus rhodochrous (R)-mandelamide
-
 ?
4.2.1.84 (R)-mandelonitrile + H2O wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% Rhodococcus rhodochrous J1 (R)-mandelamide
-
 ?
4.2.1.84 (S)-mandelonitrile + H2O wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% Rhodococcus rhodochrous (S)-mandelamide
-
 ?
4.2.1.84 (S)-mandelonitrile + H2O wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% Rhodococcus rhodochrous J1 (S)-mandelamide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.84 0.74
-
 (R)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous
4.2.1.84 1.2
-
 (S)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous
4.2.1.84 1.4
-
 (S)-mandelonitrile pH 7.4, 10°C, mutant enzyme F37H Rhodococcus rhodochrous

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.84 0.0168
-
 (R)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous
4.2.1.84 0.032
-
 (S)-mandelonitrile pH 7.4, 10°C, wild-type enzyme Rhodococcus rhodochrous
4.2.1.84 0.034
-
 (S)-mandelonitrile pH 7.4, 10°C, mutant enzyme F37H Rhodococcus rhodochrous