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Literature summary extracted from

  • Isobe, K.; Kitagawa, A.; Kanamori, K.; Kashiwagi, N.; Matsui, D.; Yamaguchi, T.; Fuhshuku, K.I.; Semba, H.; Asano, Y.
    Characterization of a novel hydroxynitrile lyase from Nandina domestica Thunb (2018), Biosci. Biotechnol. Biochem., 82, 1760-1769 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expression in Escherichia coli Nandina domestica

General Stability

EC Number General Stability Organism
4.1.2.10 the enzyme is stable without the substrate but is remarkably unstable upon incubation with benzaldehyde and KCN Nandina domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 12.5
-
 benzaldehyde pH 4.0, 25°C, hydroxynitrile lyase L Nandina domestica
4.1.2.10 19.6
-
 cyanide pH 4.0, 25°C, hydroxynitrile lyase L Nandina domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.10 Cu2+ strong inhibition, hydroxynitrile lyase L Nandina domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 24900
-
 SDS-PAGE, hydroxynitrile lyase S Nandina domestica
4.1.2.10 28000
-
 SDS-PAGE, hydroxynitrile lyase L Nandina domestica
4.1.2.10 46000 47000 gel filtration, hydroxynitrile lyase S, hydroxynitrile lyase L Nandina domestica

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Nandina domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Nandina domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 leaf the specific activity of young leaves is significantly higher than that of mature leaves Nandina domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Nandina domestica cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + 2,4-dimethylbenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2,4-dimethylphenyl)acetonitrile
-
 ?
4.1.2.10 cyanide + 2-methoxybenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2-methoxyphenyl)acetonitrile
-
 ?
4.1.2.10 cyanide + 2-methylbenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2-methylphenyl)acetonitrile
-
 ?
4.1.2.10 cyanide + 4-methoxybenzaldehyde
-
 Nandina domestica (2R)-(4-methoxyphenyl)(hydroxy)ethanenitrile
-
 ?
4.1.2.10 cyanide + 4-methylbenzaldehyde
-
 Nandina domestica (2R)-(4-methylphenyl)(hydroxy)ethanenitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde
-
 Nandina domestica (R)-mandelonitrile
-
 ?
4.1.2.10 additional information the enzyme activity toward 2-methylbenzaldehyde, 2,4-dimethylbenzaldehyde and 3-methoxybenzaldehyde is very low Nandina domestica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 NdHNL
-
 Nandina domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 15
-
 synthesis of (R)-mandelonitrile Nandina domestica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.10 5 40 5°C: about 80% of maximal activity, 40°C: about 50% of maximal activity, synthesis of (R)-mandelonitrile Nandina domestica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 55
-
 30 min, pH 6.0, without substrate, more than 80% of the original activity is retained Nandina domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 4
-
 synthesis of (R)-mandelonitrile Nandina domestica
4.1.2.10 6
-
 degradation of (R)-mandelonitrile Nandina domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 3.5 4.5 pH 3.5: about 70% of maximal activity, pH 4.5: about 75% of maximal activity, synthesis of (R)-mandelonitrile Nandina domestica
4.1.2.10 5 6.5 pH 5.0: about 70% of maximal activity, pH 6.5: about 50% of maximal activity, degradation of (R)-mandelonitrile Nandina domestica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 3.5 8 30°C, 30 min, stable Nandina domestica