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Literature summary extracted from

  • Phillips, R.S.; Ting, S.C.; Tetsadjio, A.G.; Anderson, K.L.; Friez, K.M.; Miller, K.A.; Hoover, T.R.
    Properties and mechanism of D-glucosaminate-6-phosphate ammonia-lyase An aminotransferase family enzyme with D-amino acid specificity (2018), Biochim. Biophys. Acta, 1866, 799-805 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.29 alpha-glycerophosphate weak competitive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.29 additional information no significant inhibition with D-serine at concentrations up to 200 mM Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.29 phosphate weak competitive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.29 0.14
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.29 KCl modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M) Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.29 NaCl modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M) Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.29 84000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.29 2-amino-2-deoxy-D-gluconate 6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.29 Salmonella enterica subsp. enterica serovar Typhimurium D0ZLR3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.29 2-amino-2-deoxy-D-gluconate 6-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?
4.3.1.29 2-amino-2-deoxy-D-gluconate 6-phosphate the enzyme is highly efficient and highly specific for 2-amino-2-deoxy-D-gluconate 6-phosphate. A mechanism is proposed that requires a second catalytic base located on the D-face of the substrate-pyridoxal-5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.29 dimer 2 * 39323, calculated from sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.3.1.29 DGL
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.29 27.4
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.29 7.5 8.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.29 pyridoxal 5'-phosphate a second catalytic base located on the D-face of the substrate-pyridoxal 5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.29 32
-
 alpha-glycerophosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.29 38
-
 phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.29 196
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium