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Literature summary extracted from

  • Hedge, M.; Gehring, A.; Adkins, C.; Weston, L.; Lavis, L.; Johnson, R.
    The structural basis for the narrow substrate specificity of an acetyl esterase from Thermotoga maritima (2012), Biochim. Biophys. Acta, 1824, 1024-1030 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.41 expression in Escherichia coli BL21 Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.41 S188A active site knockout Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 0.00041
-
 fluorescein dibutyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.00051
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.00054
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.00096
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.00097
-
 fluorescein dipropanoyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.001
-
 fluorescein divaleryloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.0026
-
 fluorescein dicaproyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.0037
-
 fluorescein di(acetoxymethyl ether) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.76
-
 4-nitrophenyl acetate pH 7.3, 25°C Thermotoga maritima
3.1.1.41 20.8
-
 7-aminocephalosporanic acid pH 7.3, 25°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Thermotoga maritima Q9WXT2
-
-
3.1.1.41 Thermotoga maritima DSM 3109 Q9WXT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O
-
 Thermotoga maritima DSM 3109 ?
-
 ?
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O
-
 Thermotoga maritima DSM 3109 ?
-
 ?
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O
-
 Thermotoga maritima DSM 3109 ?
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Thermotoga maritima 4-nitrophenol + acetate
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Thermotoga maritima DSM 3109 4-nitrophenol + acetate
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Thermotoga maritima acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Thermotoga maritima DSM 3109 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 fluorescein di(acetoxymethyl ether) + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 fluorescein dibutyloxymethyl ether + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 fluorescein dicaproyloxymethyl ether + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 fluorescein dipropanoyloxymethyl ether + H2O
-
 Thermotoga maritima ?
-
 ?
3.1.1.41 fluorescein divaleryloxymethyl ether + H2O
-
 Thermotoga maritima ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.41 TM0077
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 25
-
 assay at Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 7.3
-
 assay at Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.41 0.004
-
 fluorescein divaleryloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.0046
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.015
-
 fluorescein dibutyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.023
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.14
-
 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.14
-
 fluorescein dicaproyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 0.83
-
 7-aminocephalosporanic acid pH 7.3, 25°C Thermotoga maritima
3.1.1.41 6.4
-
 fluorescein dipropanoyloxymethyl ether pH 7.3, 25°C Thermotoga maritima
3.1.1.41 14.1
-
 fluorescein di(acetoxymethyl ether) pH 7.3, 25°C Thermotoga maritima
3.1.1.41 23
-
 4-nitrophenyl acetate pH 7.3, 25°C Thermotoga maritima