Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuo, T.; Liang, P.
    Reaction kinetic pathway of the recombinant octaprenyl pyrophosphate synthase from Thermotoga maritima How is it different from that of the mesophilic enzyme (2002), Biochim. Biophys. Acta, 1599, 125-133 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.90 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.90 0.0015
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C Thermotoga maritima
2.5.1.90 0.002
-
 isopentenyl diphosphate pH 7.5, 25°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Thermotoga maritima the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Thermotoga maritima DSM 3109 the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.90 Thermotoga maritima Q9X1M1
-
-
2.5.1.90 Thermotoga maritima DSM 3109 Q9X1M1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.90
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP Thermotoga maritima 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed Thermotoga maritima 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP Thermotoga maritima DSM 3109 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed Thermotoga maritima DSM 3109 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.90 octaprenyl pyrophosphate synthase
-
 Thermotoga maritima
2.5.1.90 OPPs
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.90 25 85 the enzyme activity at temperatures ranging from 25°C to 85°C exponentially increases with elevated temperature Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.90 80
-
 Tm-value is above 80°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.90 0.005
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C Thermotoga maritima
2.5.1.90 0.005
-
 isopentenyl diphosphate pH 7.5, 25°C Thermotoga maritima

General Information

EC Number General Information Comment Organism
2.5.1.90 metabolism octaprenyl pyrophosphate synthase catalyzes the chain elongation of farnesyl pyrophosphate via consecutive condensation reactions with five molecules of isopentenyl pyrophosphate to generate all-trans C40-octaprenyl pyrophosphate. The polymer forms the side chain of ubiquinone that is involved in electron transport system to produce ATP Thermotoga maritima