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Literature summary extracted from

  • Rozova, O.N.; But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Mustakhimov, I.I.; Trotsenko, Y.A.
    Characterization of two recombinant 3-hexulose-6-phosphate synthases from the halotolerant obligate methanotroph Methylomicrobium alcaliphilum 20Z (2017), Biochemistry, 82, 176-185 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed as fusion protein with 6-phospho-3-hexulose isomerase in Escherichia coli (DE3) Rosetta cells Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 ADP in the presence of 1 mM ADP, the enzyme retains 37% activity Methylotuvimicrobium alcaliphilum
4.1.2.43 AMP complete inhibition at 1 mM AMP Methylotuvimicrobium alcaliphilum
4.1.2.43 ATP in the presence of 1 mM ATP, the enzyme retains 86% activity Methylotuvimicrobium alcaliphilum
4.1.2.43 additional information not inhibited by diphosphate Methylotuvimicrobium alcaliphilum
4.1.2.43 phosphate in the presence of 1 mM phosphate, the enzyme retains 95% activity Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 0.13
-
 D-ribulose 5-phosphate at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum
4.1.2.43 0.98
-
 formaldehyde at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Mg2+ dependent on Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 40000
-
 gel filtration Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde Methylotuvimicrobium alcaliphilum
-
 D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde Methylotuvimicrobium alcaliphilum 20Z
-
 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Methylotuvimicrobium alcaliphilum
-
-
-
4.1.2.43 Methylotuvimicrobium alcaliphilum 20Z
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 Ni-NTA column chromatography Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylotuvimicrobium alcaliphilum D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylotuvimicrobium alcaliphilum 20Z D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 additional information the enzyme does not use D-glucose, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate as formaldehyde acceptors Methylotuvimicrobium alcaliphilum ?
-
 ?
4.1.2.43 additional information the enzyme does not use D-glucose, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate as formaldehyde acceptors Methylotuvimicrobium alcaliphilum 20Z ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homodimer 2 * 20000, SDS-PAGE Methylotuvimicrobium alcaliphilum
4.1.2.43 homodimer 2 * 22200, calculated from amino acid sequence Methylotuvimicrobium alcaliphilum

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 HPS
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.43 50
-
-
 Methylotuvimicrobium alcaliphilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.43 30 50 the enzyme activity is 70% higher at 50°C than at 30°C Methylotuvimicrobium alcaliphilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.43 45.82
-
 formaldehyde at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum
4.1.2.43 58.8
-
 D-ribulose 5-phosphate at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 9.5
-
-
 Methylotuvimicrobium alcaliphilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.43 46.8
-
 formaldehyde at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum
4.1.2.43 452.3
-
 D-ribulose 5-phosphate at pH 8.5 and 30°C Methylotuvimicrobium alcaliphilum