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Literature summary extracted from

  • Favrot, L.; Amorim Franco, T.M.; Blanchard, J.S.
    Biochemical characterization of the threonine deaminase (2018), Biochemistry, 57, 6003-6012 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 L-valine activator, (0-10 mM) leads to a nearly 50% increase in MsIlvA activity Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.19 expression in Escherichia coli Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.19 K67A inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme Mycolicibacterium smegmatis
4.3.1.19 K67Q inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 L-Cycloserine powerful suicide inactivator that generates a covalent pyridoxal 5'-phosphate-isoxazole complex Mycolicibacterium smegmatis
4.3.1.19 L-isoleucine less than 2% activity remains after addition of 0.5 mM L-isoleucine Mycolicibacterium smegmatis
4.3.1.19 L-valine
-
 Mycolicibacterium smegmatis
4.3.1.19 Mn2+ 50 mM, 50% loss of activity Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 1.66
-
 L-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 2.86
-
 L-allo-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 3.01
-
 beta-chloro-L-alanine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 106.3
-
 L-serine pH 8.0, 30°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.19 Ca2+ 50 mM, 1.7fold activation Mycolicibacterium smegmatis
4.3.1.19 K+ 50 mM, 1.5fold activation Mycolicibacterium smegmatis
4.3.1.19 Li+ 50 mM, 1.5fold activation Mycolicibacterium smegmatis
4.3.1.19 Mg2+ 50 mM, 1.6fold activation Mycolicibacterium smegmatis
4.3.1.19 Na+ 50 mM, 1.5fold activation Mycolicibacterium smegmatis
4.3.1.19 NH4+ 50 mM, 1.75fold activation Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 180000
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.19 L-threonine Mycolicibacterium smegmatis the enzyme is involved in the biosynthesis of L-isoleucine 2-oxobutanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Mycolicibacterium smegmatis A0QX60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 beta-chloro-L-alanine
-
 Mycolicibacterium smegmatis 3-chloro-2-oxopropionate + NH3
-
 ?
4.3.1.19 L-allo-threonine
-
 Mycolicibacterium smegmatis ?
-
 ?
4.3.1.19 L-serine
-
 Mycolicibacterium smegmatis pyruvate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Mycolicibacterium smegmatis 2-oxobutanoate + NH3
-
 ?
4.3.1.19 L-threonine the enzyme is involved in the biosynthesis of L-isoleucine Mycolicibacterium smegmatis 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.19 homotetramer
-
 Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
4.3.1.19 ilvA
-
 Mycolicibacterium smegmatis
4.3.1.19 MSMEG3183
-
 Mycolicibacterium smegmatis
4.3.1.19 Threonine deaminase
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.19 30
-
 assay at Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.19 5.69
-
 L-allo-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 9.42
-
 L-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 27.15
-
 beta-chloro-L-alanine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 32.59
-
 L-serine pH 8.0, 30°C Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 8
-
 assay at Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.19 6.8 8.9 pH has no significant effect on activity Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.19 pyridoxal 5'-phosphate dependent on Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
4.3.1.19 metabolism the enzyme is involved in the biosynthesis of L-isoleucine Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.19 0.03
-
 L-serine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 1.99
-
 L-allo-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 5.7
-
 L-threonine pH 8.0, 30°C Mycolicibacterium smegmatis
4.3.1.19 9.02
-
 beta-chloro-L-alanine pH 8.0, 30°C Mycolicibacterium smegmatis