EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.5 | expression in Escherichia coli with polyhistidine tags | Bacillus subtilis |
2.3.3.16 | expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
2.3.3.16 | expression in Escherichia coli with polyhistidine tags | Bacillus subtilis |
4.1.3.30 | expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
4.2.1.3 | expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
4.2.1.79 | expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | citrate + CoA | - |
? | |
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis 168 | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | citrate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | Bacillus subtilis 168 | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | Bacillus subtilis 168 | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | citrate + CoA | - |
? | |
2.3.3.16 | propanoyl-CoA + H2O + oxaloacetate | Bacillus subtilis | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.16 | propionyl-CoA + H2O + oxaloacetate | Bacillus subtilis | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | (2S,3S)-2-methylcitrate + CoA | - |
? | |
4.1.3.30 | 2-methylisocitrate | Bacillus subtilis | the enzyme is involved in the methylcitric acid cycle | succinate + pyruvate | - |
? | |
4.1.3.30 | 2-methylisocitrate | Bacillus subtilis 168 | the enzyme is involved in the methylcitric acid cycle | succinate + pyruvate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate | Bacillus subtilis | the enzyme is involved in the methylcitric acid cycle | 2-methylisocitrate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate | Bacillus subtilis 168 | the enzyme is involved in the methylcitric acid cycle | 2-methylisocitrate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | Escherichia coli | the enzyme is involved in the methylcitric acid cycle | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | Bacillus subtilis | the enzyme is involved in the methylcitric acid cycle | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | Bacillus subtilis 168 | the enzyme is involved in the methylcitric acid cycle | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | Escherichia coli K12 | the enzyme is involved in the methylcitric acid cycle | cis-2-methylaconitate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.5 | Bacillus subtilis | P45858 | - |
- |
2.3.3.5 | Bacillus subtilis 168 | P45858 | - |
- |
2.3.3.16 | Bacillus subtilis | P45858 | - |
- |
2.3.3.16 | Bacillus subtilis 168 | P45858 | - |
- |
4.1.3.30 | Bacillus subtilis | P54528 | - |
- |
4.1.3.30 | Bacillus subtilis 168 | P54528 | - |
- |
4.2.1.3 | Bacillus subtilis | P09339 | - |
- |
4.2.1.3 | Bacillus subtilis 168 | P09339 | - |
- |
4.2.1.79 | Bacillus subtilis | P45859 | - |
- |
4.2.1.79 | Bacillus subtilis 168 | P45859 | - |
- |
4.2.1.79 | Escherichia coli | P77243 | - |
- |
4.2.1.79 | Escherichia coli K12 | P77243 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.5 | - |
Bacillus subtilis |
2.3.3.16 | - |
Bacillus subtilis |
4.2.1.3 | - |
Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | citrate + CoA | - |
? | |
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis | citrate + CoA | - |
? | |
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis 168 | citrate + CoA | - |
? | |
2.3.3.5 | acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis 168 | citrate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis 168 | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.5 | propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) | Bacillus subtilis 168 | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + H2O + oxaloacetate | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | Bacillus subtilis 168 | citrate + CoA | - |
? | |
2.3.3.16 | propanoyl-CoA + H2O + oxaloacetate | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? | |
2.3.3.16 | propionyl-CoA + H2O + oxaloacetate | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | (2S,3S)-2-methylcitrate + CoA | - |
? | |
2.3.3.16 | propionyl-CoA + H2O + oxaloacetate | MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis | (2S,3S)-2-methylcitrate + CoA | - |
? | |
4.1.3.30 | 2-methylisocitrate | - |
Bacillus subtilis | succinate + pyruvate | - |
? | |
4.1.3.30 | 2-methylisocitrate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | succinate + pyruvate | - |
? | |
4.1.3.30 | 2-methylisocitrate | - |
Bacillus subtilis 168 | succinate + pyruvate | - |
? | |
4.1.3.30 | 2-methylisocitrate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis 168 | succinate + pyruvate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | 2-methylisocitrate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis 168 | 2-methylisocitrate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate + H2O | - |
Bacillus subtilis | 2-methylisocitrate | - |
? | |
4.2.1.3 | (Z)-2-methylaconitate + H2O | - |
Bacillus subtilis 168 | 2-methylisocitrate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the enzyme is involved in the methylcitric acid cycle | Escherichia coli | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate | Escherichia coli | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate | Bacillus subtilis | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis 168 | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate | Bacillus subtilis 168 | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the enzyme is involved in the methylcitric acid cycle | Escherichia coli K12 | cis-2-methylaconitate | - |
? | |
4.2.1.79 | (2S,3S)-2-methylcitrate | the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate | Escherichia coli K12 | cis-2-methylaconitate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.5 | citrate synthase/2-methylcitrate synthase | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis |
2.3.3.5 | mmgD | bifunctional citrate/2-methylcitrate synthase | Bacillus subtilis |
2.3.3.16 | bifunctional citrate synthase/2-methylcitrate synthase | - |
Bacillus subtilis |
2.3.3.16 | citrate synthase/2-methylcitrate synthase | bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase | Bacillus subtilis |
2.3.3.16 | mmgD | - |
Bacillus subtilis |
2.3.3.16 | mmgD | bifunctional citrate/2-methylcitrate synthase | Bacillus subtilis |
4.1.3.30 | 2-Methylisocitrate lyase | - |
Bacillus subtilis |
4.1.3.30 | mmgF | - |
Bacillus subtilis |
4.1.3.30 | yqiQ | - |
Bacillus subtilis |
4.2.1.3 | aconitase/2-methylaconitate hydratase | - |
Bacillus subtilis |
4.2.1.3 | citB | - |
Bacillus subtilis |
4.2.1.79 | 2-methylcitrate dehydratase | - |
Escherichia coli |
4.2.1.79 | 2-methylcitrate dehydratase | - |
Bacillus subtilis |
4.2.1.79 | mmgE | - |
Bacillus subtilis |
4.2.1.79 | PrpD | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.3 | 25 | - |
assay at | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.3 | 7.5 | - |
assay at | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.3 | iron-sulfur centre | - |
Bacillus subtilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.3.5 | metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |
2.3.3.16 | metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |
4.1.3.30 | metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |
4.2.1.3 | metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |
4.2.1.79 | metabolism | the enzyme is involved in the methylcitric acid cycle | Escherichia coli |
4.2.1.79 | metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |