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Literature summary extracted from

  • Reddick, J.J.; Sirkisoon, S.; Dahal, R.A.; Hardesty, G.; Hage, N.E.; Booth, W.T.; Quattlebaum, A.L.; Mills, S.N.; Meadows, V.G.; Adams, S.L.H.; Doyle, J.S.; Kiel, B.E.
    First biochemical characterization of a methylcitric acid cycle from Bacillus subtilis strain 168 (2017), Biochemistry, 56, 5698-5711 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 expression in Escherichia coli with polyhistidine tags Bacillus subtilis
2.3.3.16 expression in Escherichia coli BL21(DE3) Bacillus subtilis
2.3.3.16 expression in Escherichia coli with polyhistidine tags Bacillus subtilis
4.1.3.30 expression in Escherichia coli BL21(DE3) Bacillus subtilis
4.2.1.3 expression in Escherichia coli BL21(DE3) Bacillus subtilis
4.2.1.79 expression in Escherichia coli BL21(DE3) Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase citrate + CoA
-
?
2.3.3.5 acetyl-CoA + H2O + oxaloacetate Bacillus subtilis 168 bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase citrate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Bacillus subtilis 168 bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Bacillus subtilis MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle citrate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase citrate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Bacillus subtilis 168 MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle citrate + CoA
-
?
2.3.3.16 propanoyl-CoA + H2O + oxaloacetate Bacillus subtilis bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.16 propionyl-CoA + H2O + oxaloacetate Bacillus subtilis MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle (2S,3S)-2-methylcitrate + CoA
-
?
4.1.3.30 2-methylisocitrate Bacillus subtilis the enzyme is involved in the methylcitric acid cycle succinate + pyruvate
-
?
4.1.3.30 2-methylisocitrate Bacillus subtilis 168 the enzyme is involved in the methylcitric acid cycle succinate + pyruvate
-
?
4.2.1.3 (Z)-2-methylaconitate Bacillus subtilis the enzyme is involved in the methylcitric acid cycle 2-methylisocitrate
-
?
4.2.1.3 (Z)-2-methylaconitate Bacillus subtilis 168 the enzyme is involved in the methylcitric acid cycle 2-methylisocitrate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate Escherichia coli the enzyme is involved in the methylcitric acid cycle cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate Bacillus subtilis the enzyme is involved in the methylcitric acid cycle cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate Bacillus subtilis 168 the enzyme is involved in the methylcitric acid cycle cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate Escherichia coli K12 the enzyme is involved in the methylcitric acid cycle cis-2-methylaconitate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Bacillus subtilis P45858
-
-
2.3.3.5 Bacillus subtilis 168 P45858
-
-
2.3.3.16 Bacillus subtilis P45858
-
-
2.3.3.16 Bacillus subtilis 168 P45858
-
-
4.1.3.30 Bacillus subtilis P54528
-
-
4.1.3.30 Bacillus subtilis 168 P54528
-
-
4.2.1.3 Bacillus subtilis P09339
-
-
4.2.1.3 Bacillus subtilis 168 P09339
-
-
4.2.1.79 Bacillus subtilis P45859
-
-
4.2.1.79 Bacillus subtilis 168 P45859
-
-
4.2.1.79 Escherichia coli P77243
-
-
4.2.1.79 Escherichia coli K12 P77243
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5
-
Bacillus subtilis
2.3.3.16
-
Bacillus subtilis
4.2.1.3
-
Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis citrate + CoA
-
?
2.3.3.5 acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis citrate + CoA
-
?
2.3.3.5 acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis 168 citrate + CoA
-
?
2.3.3.5 acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis 168 citrate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis 168 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16) Bacillus subtilis 168 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle Bacillus subtilis citrate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase Bacillus subtilis citrate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis citrate + CoA
-
?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle Bacillus subtilis 168 citrate + CoA
-
?
2.3.3.16 propanoyl-CoA + H2O + oxaloacetate bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
2.3.3.16 propionyl-CoA + H2O + oxaloacetate MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase. The enzyme is involved in the methylcitric acid cycle Bacillus subtilis (2S,3S)-2-methylcitrate + CoA
-
?
2.3.3.16 propionyl-CoA + H2O + oxaloacetate MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3fold higher activity as a 2-methylcitrate synthase Bacillus subtilis (2S,3S)-2-methylcitrate + CoA
-
?
4.1.3.30 2-methylisocitrate
-
Bacillus subtilis succinate + pyruvate
-
?
4.1.3.30 2-methylisocitrate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis succinate + pyruvate
-
?
4.1.3.30 2-methylisocitrate
-
Bacillus subtilis 168 succinate + pyruvate
-
?
4.1.3.30 2-methylisocitrate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis 168 succinate + pyruvate
-
?
4.2.1.3 (Z)-2-methylaconitate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis 2-methylisocitrate
-
?
4.2.1.3 (Z)-2-methylaconitate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis 168 2-methylisocitrate
-
?
4.2.1.3 (Z)-2-methylaconitate + H2O
-
Bacillus subtilis 2-methylisocitrate
-
?
4.2.1.3 (Z)-2-methylaconitate + H2O
-
Bacillus subtilis 168 2-methylisocitrate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the enzyme is involved in the methylcitric acid cycle Escherichia coli cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate Escherichia coli cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate Bacillus subtilis cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the enzyme is involved in the methylcitric acid cycle Bacillus subtilis 168 cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate Bacillus subtilis 168 cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the enzyme is involved in the methylcitric acid cycle Escherichia coli K12 cis-2-methylaconitate
-
?
4.2.1.79 (2S,3S)-2-methylcitrate the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate Escherichia coli K12 cis-2-methylaconitate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 citrate synthase/2-methylcitrate synthase bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis
2.3.3.5 mmgD bifunctional citrate/2-methylcitrate synthase Bacillus subtilis
2.3.3.16 bifunctional citrate synthase/2-methylcitrate synthase
-
Bacillus subtilis
2.3.3.16 citrate synthase/2-methylcitrate synthase bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase Bacillus subtilis
2.3.3.16 mmgD
-
Bacillus subtilis
2.3.3.16 mmgD bifunctional citrate/2-methylcitrate synthase Bacillus subtilis
4.1.3.30 2-Methylisocitrate lyase
-
Bacillus subtilis
4.1.3.30 mmgF
-
Bacillus subtilis
4.1.3.30 yqiQ
-
Bacillus subtilis
4.2.1.3 aconitase/2-methylaconitate hydratase
-
Bacillus subtilis
4.2.1.3 citB
-
Bacillus subtilis
4.2.1.79 2-methylcitrate dehydratase
-
Escherichia coli
4.2.1.79 2-methylcitrate dehydratase
-
Bacillus subtilis
4.2.1.79 mmgE
-
Bacillus subtilis
4.2.1.79 PrpD
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.3 25
-
assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 7.5
-
assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.3 iron-sulfur centre
-
Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.3.3.5 metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis
2.3.3.16 metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis
4.1.3.30 metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis
4.2.1.3 metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis
4.2.1.79 metabolism the enzyme is involved in the methylcitric acid cycle Escherichia coli
4.2.1.79 metabolism the enzyme is involved in the methylcitric acid cycle Bacillus subtilis