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Literature summary extracted from

  • Maneiro, M.; Peon, A.; Lence, E.; Otero, J.M.; Van Raaij, M.J.; Thompson, P.; Hawkins, A.R.; Gonzalez-Bello, C.
    Insights into substrate binding and catalysis in bacterial type I dehydroquinase (2014), Biochem. J., 462, 415-424 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 crystal structure in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, at 1.5 A. Residues Gln236, Pro234 and Ala233 are located in the flexible substrate-covering loop. Gln236 is responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 is important in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis Mycobacterium tuberculosis
4.2.1.10 crystal structure in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, at 1.5 A. Residues Gln236, Pro234 and Ala233 are located in the flexible substrate-covering loop. Gln236 is responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 is important in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis Salmonella enterica subsp. enterica serovar Typhi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.10 (2R)-2-methyl-3-dehydroquinic acid compound is a reversible competive inhibitor of type 1 enzymes and a weak substrate of type 2 enzymes Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.10 0.011
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
4.2.1.10 0.015
-
 3-dehydroquinate pH 7.2, 25°C Mycobacterium tuberculosis
4.2.1.10 0.024
-
 3-dehydroquinate pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Mycobacterium tuberculosis
-
-
-
4.2.1.10 Salmonella enterica subsp. enterica serovar Typhi P24670
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 (2R)-2-methyl-3-dehydroquinic acid compound is a reversible competive inhibitor of type 1 enzymes and a weak substrate of type 2 enzymes Mycobacterium tuberculosis ?
-
 ?
4.2.1.10 3-dehydroquinate
-
 Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate
-
 Salmonella enterica subsp. enterica serovar Typhi 3-dehydroshikimate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 aroD
-
 Mycobacterium tuberculosis
4.2.1.10 aroD
-
 Salmonella enterica subsp. enterica serovar Typhi
4.2.1.10 DHQ1
-
 Salmonella enterica subsp. enterica serovar Typhi
4.2.1.10 DHQ2
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.10 0.66
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
4.2.1.10 1.1
-
 3-dehydroquinate pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi
4.2.1.10 154
-
 3-dehydroquinate pH 7.0, 25°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.10 0.305
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.10 60
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
4.2.1.10 10270
-
 3-dehydroquinate pH 7.2, 25°C Mycobacterium tuberculosis