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Literature summary extracted from

  • Wang, J.; Chen, C.C.; Yang, Y.; Liu, W.; Ko, T.P.; Shang, N.; Hu, X.; Xie, Y.; Huang, J.W.; Zhang, Y.; Guo, R.T.
    Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis (2018), Biochem. Biophys. Res. Commun., 495, 1782-1788 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.111 expressed in Escherichia coli BL21(DE3) cells Fischerella ambigua

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.111 sitting drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M imidazole, pH 8.0 Fischerella ambigua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate Fischerella ambigua preferred reaction 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole
-
 ?
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate Fischerella ambigua UTEX 1903 preferred reaction 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.111 Fischerella ambigua A0A1J0AKI6
-
-
2.5.1.111 Fischerella ambigua UTEX 1903 A0A1J0AKI6
-
-
2.5.1.146 Fischerella ambigua V5TDZ4
-
-
2.5.1.146 Fischerella ambigua UTEX 1903 V5TDZ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.111 Ni-NTA column chromatography Fischerella ambigua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile dimethylallyl diphosphate is preferred over geranyl diphosphate Fischerella ambigua diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile
-
 ?
2.5.1.111 dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile dimethylallyl diphosphate is preferred over geranyl diphosphate Fischerella ambigua UTEX 1903 diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile
-
 ?
2.5.1.111 dimethylallyl diphosphate + hapalindole U
-
 Fischerella ambigua diphosphate + ambiguine H
-
 ?
2.5.1.111 dimethylallyl diphosphate + hapalindole U
-
 Fischerella ambigua UTEX 1903 diphosphate + ambiguine H
-
 ?
2.5.1.111 geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile dimethylallyl diphosphate is preferred over geranyl diphosphate Fischerella ambigua diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile
-
 ?
2.5.1.111 geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile dimethylallyl diphosphate is preferred over geranyl diphosphate Fischerella ambigua UTEX 1903 diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile
-
 ?
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + dimethylallyl diphosphate
-
 Fischerella ambigua 3-[(E)-2-isocyanoethenyl]-2-(3-methylbut-2-en-1-yl)-1H-indole + 3-[(E)-2-isocyanoethenyl]-3-(3-methylbut-2-en-1-yl)-3H-indole
-
 ?
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + dimethylallyl diphosphate
-
 Fischerella ambigua UTEX 1903 3-[(E)-2-isocyanoethenyl]-2-(3-methylbut-2-en-1-yl)-1H-indole + 3-[(E)-2-isocyanoethenyl]-3-(3-methylbut-2-en-1-yl)-3H-indole
-
 ?
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate preferred reaction Fischerella ambigua 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole
-
 ?
2.5.1.146 3-[(E)-2-isocyanoethenyl]-1H-indole + geranyl diphosphate preferred reaction Fischerella ambigua UTEX 1903 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-1H-indole + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3-[(Z)-2-isocyanoethenyl]-3H-indole
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.111 apT
-
 Fischerella ambigua
2.5.1.111 aromatic prenyltransferase
-
 Fischerella ambigua
2.5.1.111 FamD1
-
 Fischerella ambigua
2.5.1.146 famD2
-
 Fischerella ambigua