Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toyomasu, T.; Miyamoto, K.; Shenton, M.R.; Sakai, A.; Sugawara, C.; Horie, K.; Kawaide, H.; Hasegawa, M.; Chuba, M.; Mitsuhashi, W.; Yamane, H.; Kurata, N.; Okada, K.
    Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon (2016), Biochem. Biophys. Res. Commun., 480, 402-408 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.29 gene KSL10, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group
4.2.3.29 gene KSL10i, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.29 gene KSL10j, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.30 gene KSL5, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.30 gene KSL5j, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group
4.2.3.33 gene KSL8, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group
4.2.3.33 gene KSL8ind, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.34 gene KSL8, sequence comparisons and phylogenetic analysis and tree Oryza sativa Indica Group
4.2.3.34 gene KSL8ind, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.103 gene OrKSL5ind, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.103 gene OrKSL6, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.103 gene OrKSL6ind, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
4.2.3.103 gene OsKSL5i, sequence comparisons and phylogenetic analysis and tree Oryza sativa Indica Group
4.2.3.103 gene OsKSL6, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.103 chloroplast
-
 Oryza rufipogon 9507
-
4.2.3.103 chloroplast
-
 Oryza sativa Japonica Group 9507
-
4.2.3.103 chloroplast
-
 Oryza sativa Indica Group 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.29 Mg2+ required Oryza rufipogon
4.2.3.29 Mg2+ required Oryza sativa Japonica Group
4.2.3.30 Mg2+ required Oryza sativa Japonica Group
4.2.3.30 Mg2+ required Oryza rufipogon
4.2.3.33 Mg2+ required Oryza rufipogon
4.2.3.33 Mg2+ required Oryza sativa Japonica Group
4.2.3.34 Mg2+ required Oryza rufipogon
4.2.3.34 Mg2+ required Oryza sativa Indica Group
4.2.3.103 Mg2+ required Oryza rufipogon
4.2.3.103 Mg2+ required Oryza sativa Japonica Group
4.2.3.103 Mg2+ required Oryza sativa Indica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.29 ent-copalyl diphosphate Oryza rufipogon
-
 ent-sandaracopimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.29 ent-copalyl diphosphate Oryza sativa Japonica Group
-
 ent-sandaracopimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.30 ent-copalyl diphosphate Oryza sativa Japonica Group
-
 ent-pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.30 ent-copalyl diphosphate Oryza rufipogon
-
 ent-pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.33 9alpha-copalyl diphosphate Oryza rufipogon
-
 stemod-13-ene + diphosphate
-
 ?
4.2.3.33 9alpha-copalyl diphosphate Oryza sativa Japonica Group
-
 stemod-13-ene + diphosphate
-
 ?
4.2.3.34 9alpha-copalyl diphosphate Oryza rufipogon
-
 stemod-13(17)-ene + diphosphate
-
 ?
4.2.3.34 9alpha-copalyl diphosphate Oryza sativa Indica Group
-
 stemod-13(17)-ene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate Oryza rufipogon
-
 ent-isokaurene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate Oryza sativa Japonica Group
-
 ent-isokaurene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate Oryza sativa Indica Group
-
 ent-isokaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.29 Oryza rufipogon A0A1L7NUB5 cv. W106
-
4.2.3.29 Oryza rufipogon A0A2Z6BAT9 cv. W1943
-
4.2.3.29 Oryza sativa Japonica Group Q2QQJ5
-
-
4.2.3.30 Oryza rufipogon LC169117 cv. W0106
-
4.2.3.30 Oryza sativa Japonica Group Q6Z5J6
-
-
4.2.3.33 Oryza rufipogon A0A2Z6BAJ2 cv. W01943
-
4.2.3.33 Oryza sativa Japonica Group Q6BDZ9 cv. Nipponbare
-
4.2.3.34 Oryza rufipogon A0A2Z6BAT9 cv. W0106
-
4.2.3.34 Oryza sativa Indica Group A0A2Z6BAT9
-
-
4.2.3.103 Oryza rufipogon
-
-
-
4.2.3.103 Oryza rufipogon LC169118
-
-
4.2.3.103 Oryza sativa Indica Group A4KAG7
-
-
4.2.3.103 Oryza sativa Japonica Group A4KAG8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.29 leaf
-
 Oryza rufipogon
-
4.2.3.29 leaf
-
 Oryza sativa Japonica Group
-
4.2.3.30 leaf
-
 Oryza sativa Japonica Group
-
4.2.3.30 leaf
-
 Oryza rufipogon
-
4.2.3.33 leaf
-
 Oryza rufipogon
-
4.2.3.33 leaf
-
 Oryza sativa Japonica Group
-
4.2.3.34 leaf
-
 Oryza rufipogon
-
4.2.3.34 leaf
-
 Oryza sativa Indica Group
-
4.2.3.103 leaf
-
 Oryza rufipogon
-
4.2.3.103 leaf
-
 Oryza sativa Japonica Group
-
4.2.3.103 leaf
-
 Oryza sativa Indica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.29 ent-copalyl diphosphate
-
 Oryza rufipogon ent-sandaracopimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.29 ent-copalyl diphosphate
-
 Oryza sativa Japonica Group ent-sandaracopimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.30 ent-copalyl diphosphate
-
 Oryza sativa Japonica Group ent-pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.30 ent-copalyl diphosphate
-
 Oryza rufipogon ent-pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.33 9alpha-copalyl diphosphate
-
 Oryza rufipogon stemod-13-ene + diphosphate
-
 ?
4.2.3.33 9alpha-copalyl diphosphate
-
 Oryza sativa Japonica Group stemod-13-ene + diphosphate
-
 ?
4.2.3.33 9alpha-copalyl diphosphate i.e. syn-copalyl diphosphate Oryza sativa Japonica Group stemod-13-ene + diphosphate
-
 ?
4.2.3.34 9alpha-copalyl diphosphate
-
 Oryza rufipogon stemod-13(17)-ene + diphosphate
-
 ?
4.2.3.34 9alpha-copalyl diphosphate
-
 Oryza sativa Indica Group stemod-13(17)-ene + diphosphate
-
 ?
4.2.3.34 9alpha-copalyl diphosphate i.e. syn-copalyl diphosphate Oryza sativa Indica Group stemod-13(17)-ene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate
-
 Oryza rufipogon ent-isokaurene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate
-
 Oryza sativa Japonica Group ent-isokaurene + diphosphate
-
 ?
4.2.3.103 ent-copalyl diphosphate
-
 Oryza sativa Indica Group ent-isokaurene + diphosphate
-
 ?
4.2.3.103 additional information recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene Oryza rufipogon ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.29 ent-kaurene synthase like 10 UniProt Oryza rufipogon
4.2.3.29 ent-sandaracopimaradiene synthase
-
 Oryza rufipogon
4.2.3.29 ent-sandaracopimaradiene synthase
-
 Oryza sativa Japonica Group
4.2.3.29 KS like 10
-
 Oryza sativa Japonica Group
4.2.3.29 KSL10
-
 Oryza sativa Japonica Group
4.2.3.29 KSL10i
-
 Oryza rufipogon
4.2.3.29 KSL10j
-
 Oryza rufipogon
4.2.3.29 OrKSL10i
-
 Oryza rufipogon
4.2.3.29 OrKSL10j
-
 Oryza rufipogon
4.2.3.29 OsKSL10
-
 Oryza sativa Japonica Group
4.2.3.30 ent-kaurene synthase-like 5 UniProt Oryza sativa Japonica Group
4.2.3.30 KSL5
-
 Oryza sativa Japonica Group
4.2.3.30 KSL5
-
 Oryza rufipogon
4.2.3.30 OrKSL5
-
 Oryza rufipogon
4.2.3.30 OsKSL5j
-
 Oryza sativa Japonica Group
4.2.3.33 ent-kaurene synthase like 8 japonica UniProt Oryza rufipogon
4.2.3.33 ent-kaurene synthase-like 8
-
 Oryza sativa Japonica Group
4.2.3.33 KS like 8
-
 Oryza rufipogon
4.2.3.33 KSL8
-
 Oryza sativa Japonica Group
4.2.3.33 OrKSL8j
-
 Oryza rufipogon
4.2.3.33 OsKSL8
-
 Oryza sativa Japonica Group
4.2.3.33 stemar-13-ene synthase
-
 Oryza sativa Japonica Group
4.2.3.33 stemod-13-ene synthase
-
 Oryza rufipogon
4.2.3.34 ent-kaurene synthase like 8 indica UniProt Oryza rufipogon
4.2.3.34 KS like 8
-
 Oryza rufipogon
4.2.3.34 KS like 8
-
 Oryza sativa Indica Group
4.2.3.34 KSL11 UniProt Oryza sativa Indica Group
4.2.3.34 KSL8
-
 Oryza sativa Indica Group
4.2.3.34 OrKSL8ind
-
 Oryza rufipogon
4.2.3.34 OsKSL8ind
-
 Oryza sativa Indica Group
4.2.3.34 stemod-13(17)-ene synthase
-
 Oryza rufipogon
4.2.3.34 stemod-13(17)-ene synthase
-
 Oryza sativa Indica Group
4.2.3.103 ent-kaurene synthase like
-
 Oryza rufipogon
4.2.3.103 ent-kaurene synthase like
-
 Oryza sativa Indica Group
4.2.3.103 ent-kaurene synthase like 5
-
 Oryza rufipogon
4.2.3.103 ent-kaurene synthase like 6
-
 Oryza sativa Japonica Group
4.2.3.103 KSL5i
-
 Oryza sativa Indica Group
4.2.3.103 KSL5ind
-
 Oryza rufipogon
4.2.3.103 KSL6
-
 Oryza sativa Japonica Group
4.2.3.103 KSL6
-
 Oryza rufipogon
4.2.3.103 KSL6ind
-
 Oryza rufipogon
4.2.3.103 OrKSL5ind
-
 Oryza rufipogon
4.2.3.103 OrKSL6
-
 Oryza rufipogon
4.2.3.103 OrKSL6ind
-
 Oryza rufipogon
4.2.3.103 OsKSL5i
-
 Oryza sativa Indica Group
4.2.3.103 OsKSL6
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
4.2.3.29 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
4.2.3.29 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza sativa Japonica Group
4.2.3.29 metabolism LC-MS/MS analyses show that oryzalexins A and C are not detected in either accession W0106 or W1943. Oryzalexins E and F, which are not detected in the two Or accessions, are also undetectable in Oryza sativa cv. Nipponbare. OrKSL10 and OrKSL10ind cannot usually produce ent-sandaracopimaradiene strongly suggesting that no accumulation of oryzalexins A-F is possible in either of the two Or accessions Oryza rufipogon
4.2.3.29 additional information recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity Oryza rufipogon
4.2.3.29 physiological function OrKSL10j and OrKSL10ind encode ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F Oryza rufipogon
4.2.3.29 physiological function OsKSL10 encodes ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F, but oryzalexins E and F are undetectable in Oryza sativa cv. Nipponbare, they are also undetectable in Oryza rufipogon accessions W106 and W1943 Oryza sativa Japonica Group
4.2.3.30 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
4.2.3.30 evolution enzymes KSLs belong to the type A/class I terpene synthase family. The KSL5 members are clearly divided into two clades, representing japonica and indica groups. The KS responsible for gibberellin biosynthesis in a putative common ancestor, and OsKS1, which produces the tetracyclic ent-kaurene, has Ile as the corresponding key amino acid. Thus, it is suggested that substitution from Ile664 to Thr664 in KSL5 occurred before the onset of cultivation in the putative japonica ancestor. The OsKSL5 sequence is similar to the OsKSL6 sequence (89% identity), and it is therefore likely that the two KSL genes were generated through relatively recent duplication. The substitution in KSL5 probably occurred after duplication of the ancestral gene Oryza sativa Japonica Group
4.2.3.30 physiological function recombinant OrKSL5 converts ent-CDP to ent-pimara-8(14),15-diene (EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-CDP to ent-isokaurene (EC 4.2.3.103). The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively, as is the case for Oryza sativa OsKSL5 and OsKSL5ind Oryza rufipogon
4.2.3.30 physiological function the KSL5 members are clearly divided into two clades, representing japonica and indica groups, the 664th amino acids of OsKSL5j and OsKSL5i are Thr and Ile leading to different activity and production of ent-isokeurene synthase (EC 4.2.3.103) or ent-pimara-8(14),15-diene (EC 4.2.3.30) Oryza sativa Japonica Group
4.2.3.33 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
4.2.3.33 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza sativa Japonica Group
4.2.3.33 physiological function OrKSL8j functions as a stemod-13-ene synthase responsible for biosynthesis of oryzalexins S Oryza rufipogon
4.2.3.33 physiological function OsKSL8 encodes a stemar-13-ene synthase responsible for biosynthesis of oryzalexins S Oryza sativa Japonica Group
4.2.3.34 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
4.2.3.34 evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza sativa Indica Group
4.2.3.34 physiological function OrKSL8ind functions as a stemod-13(17)-ene synthase not as a stemar-13-ene synthase Oryza rufipogon
4.2.3.34 physiological function OsKSL8 encodes a stemar-13(17)-ene synthase Oryza sativa Indica Group
4.2.3.103 evolution enzymes KSLs belong to the type A/class I terpene synthase family. The KSL5 members are clearly divided into two clades, representing japonica and indica groups. The KS responsible for gibberellin biosynthesis in a putative common ancestor, and OsKS1, which produces the tetracyclic ent-kaurene, has Ile as the corresponding key amino acid. Thus, it is suggested that substitution from Ile664 to Thr664 in KSL5 occurred before the onset of cultivation in the putative japonica ancestor. The OsKSL5 sequence is similar to the OsKSL6 sequence (89% identity), and it is therefore likely that the two KSL genes were generated through relatively recent duplication. The substitution in KSL5 probably occurred after duplication of the ancestral gene Oryza sativa Indica Group
4.2.3.103 physiological function OsKSL5i and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones Oryza sativa Japonica Group
4.2.3.103 physiological function OsKSL5i and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones. The KSL5 members are clearly divided into two clades, representing japonica and indica groups, the 664th amino acids of OsKSL5j and OsKSL5i are Thr and Ile leading to different activity and production of ent-isokeurene synthase (EC 4.2.3.103) or ent-pimara-8(14),15-diene (EC 4.2.3.30) Oryza sativa Indica Group
4.2.3.103 physiological function OsKSL5ind and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones. Recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene. The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively Oryza rufipogon
4.2.3.103 physiological function recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene Oryza rufipogon
4.2.3.103 physiological function recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene. The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively Oryza rufipogon