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Literature summary extracted from

  • Tezuka, D.; Ito, A.; Mitsuhashi, W.; Toyomasu, T.; Imai, R.
    The rice ent-kaurene synthase like 2 encodes a functional ent-beyerene synthase (2015), Biochem. Biophys. Res. Commun., 460, 766-771 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.19 expressed in Escherichia coli BL21 cells Oryza sativa
4.2.3.B66 gene OsKSL2, full-length enzyme DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.B66 additional information construction of a recombinant gene where GFP is fused to the putative chloroplast transit peptide (cTP) sequence of OsKSL2. The fusion gene is transiently expressed in rice leaf protoplasts. GFP signals from the transformed cells overlap with autofluorescence from chloroplasts, indicating that OsKSL2 is localized to the plastid. The subcellular localization of the recombinant GFP-tagged enzyme with GFP fused to a shorter transit peptide sequence from gene Os04g0612000 (UniProt ID Q0JA81) in protoplast cells does not localize to the plastids, the shorter sequence fails to function as a transit peptide Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.B66 chloroplast the first 58 residues of OsKSL2 function as a putative chloroplast transit peptide Oryza sativa Japonica Group 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.B66 Mg2+ enzyme-bound Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Oryza sativa
-
 ent-beyerene + ent-kaurene + diphosphate
-
 ?
4.2.3.B66 ent-copalyl diphosphate Oryza sativa Japonica Group
-
 ent-beyerene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Oryza sativa
-
 cultivar Yukihikari
-
4.2.3.B66 Oryza sativa Japonica Group A0A0F7QZZ2 cv. Yukihikari
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.19 glutathione-affinity column chromatography Oryza sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.B66 ent-copalyl diphosphate = ent-beyerene + diphosphate reaction mechanism via carbocation intermediate, in comparison to ant-kaurene synthesis from ent-copalyl diphosphate (EC 4.2.3.19), mass spectrometric analysis Oryza sativa Japonica Group
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.19 root
-
 Oryza sativa
-
4.2.3.B66 root
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Oryza sativa ent-beyerene + ent-kaurene + diphosphate
-
 ?
4.2.3.B66 ent-copalyl diphosphate
-
 Oryza sativa Japonica Group ent-beyerene + diphosphate
-
 ?
4.2.3.B66 additional information recombinant OsKSL2 enzyme catalyzes the cyclization of ent-copalyl diphosphate into ent-beyerene as a major and ent-kaurene as a minor product, cf. EC 4.2.3.19, GC-MS product analysis Oryza sativa Japonica Group ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 ent-beyerene synthase
-
 Oryza sativa
4.2.3.19 ent-kaurene synthase like 2
-
 Oryza sativa
4.2.3.19 KSL2
-
 Oryza sativa
4.2.3.B66 ent-kaurene synthase like 2
-
 Oryza sativa Japonica Group
4.2.3.B66 OsKSL2
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
4.2.3.19 Oryza sativa kaurene synthase-like 2 is drastically induced in roots by 0.1 mM methyl jasmonate treatment up
4.2.3.B66 Oryza sativa Japonica Group OsKSL2 is induced by methyl jasmonate up

General Information

EC Number General Information Comment Organism
4.2.3.19 physiological function the enzyme is involved in defense mechanisms in rice roots Oryza sativa
4.2.3.B66 evolution the enzyme belongs to the class I diterpene synthases Oryza sativa Japonica Group
4.2.3.B66 physiological function OsKSL2 is a functional ent-beyerene synthase that is involved in defense mechanisms in rice roots. ent-Beyerene is an antimicrobial compound Oryza sativa Japonica Group