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Literature summary extracted from

  • Sousa, S.A.; Moreira, L.M.; Wopperer, J.; Eberl, L.; Sa-Correira, I.; Leitao, J.H.
    The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expression in Escherichia coli Burkholderia cepacia
2.7.7.13 expression in of the His-tagged enzyme in Escherichia coli Burkholderia cepacia
5.3.1.8 expression of the His-tagged enzyme in Escherichia coli Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 EDTA no activity is detected when 2 mM EDTA is added to the dialysis buffer Burkholderia cepacia
5.3.1.8 EDTA no activity is detected when 2 mM EDTA is added to the dialysis buffer Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 2.9
-
 GDP-mannose pH 7.6, temperature not specified in the publication Burkholderia cepacia
2.7.7.13 2.9
-
 GDP-alpha-D-mannose pH 7.6, 10 mM Mn2+, temperature not specified in the publication Burkholderia cepacia
5.3.1.8 9.01
-
 D-mannose 6-phosphate pH 7.6, 5 mM Mg2+, temperature not specified in the publication Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Ca2+ GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ Burkholderia cepacia
2.7.7.13 Ca2+ GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
2.7.7.13 Mn2+ GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ Burkholderia cepacia
2.7.7.13 Mn2+ GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
5.3.1.8 Ca2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
5.3.1.8 Co2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
5.3.1.8 Mg2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
5.3.1.8 Mn2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
5.3.1.8 Ni2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 55000
-
 SDS-PAGE Burkholderia cepacia
2.7.7.13 55300
-
 calculated from sequence Burkholderia cepacia
5.3.1.8 55000
-
 SDS-PAGE Burkholderia cepacia
5.3.1.8 55300
-
 calculated from sequence Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Burkholderia cepacia Q06XM8
-
-
2.7.7.13 Burkholderia cepacia IST408 Q06XM8
-
-
5.3.1.8 Burkholderia cepacia Q06XM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13
-
 Burkholderia cepacia
5.3.1.8
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 diphosphate + GDP-alpha-D-mannose
-
 Burkholderia cepacia GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 diphosphate + GDP-alpha-D-mannose
-
 Burkholderia cepacia IST408 GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 diphosphate + GDP-mannose
-
 Burkholderia cepacia GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 diphosphate + GDP-mannose
-
 Burkholderia cepacia IST408 GTP + alpha-D-mannose 1-phosphate
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Burkholderia cepacia D-Fructose 6-phosphate
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Burkholderia cepacia IST408 D-Fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ? x * 55000, SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 BceA
-
 Burkholderia cepacia
2.7.7.13 GDP-mannose pyrophosphorylase
-
 Burkholderia cepacia
2.7.7.13 More bifunctional protein (type II PMI family) with phosphomannose isomerase (PMI, EC 5.3.1.8) and GDP-mannose pyrophosphorylase (GMP, EC 2.7.7.13) activities Burkholderia cepacia
5.3.1.8 BceA
-
 Burkholderia cepacia
5.3.1.8 Phosphomannose isomerase
-
 Burkholderia cepacia
5.3.1.8 PMI
-
 Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.13 195.9
-
 GDP-alpha-D-mannose pH 7.6, 10 mM Mn2+, temperature not specified in the publication Burkholderia cepacia
5.3.1.8 19.4
-
 D-mannose 6-phosphate pH 7.6, 5 mM Mg2+, temperature not specified in the publication Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 7.6
-
 assay at Burkholderia cepacia
5.3.1.8 7.6
-
 assay at Burkholderia cepacia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.13 Burkholderia cepacia calculated from sequence
-
 6.03
5.3.1.8 Burkholderia cepacia calculated from sequence
-
 6.03

General Information

EC Number General Information Comment Organism
2.7.7.13 malfunction the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation Burkholderia cepacia
2.7.7.13 metabolism the bceA gene is part of the exopolysaccharide biosynthetic cluster Burkholderia cepacia
2.7.7.13 physiological function the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed Burkholderia cepacia
5.3.1.8 malfunction the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation Burkholderia cepacia
5.3.1.8 physiological function the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed Burkholderia cepacia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.13 195.9
-
 GDP-mannose pH 7.6, temperature not specified in the publication Burkholderia cepacia