EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.13 | expression in Escherichia coli | Burkholderia cepacia |
2.7.7.13 | expression in of the His-tagged enzyme in Escherichia coli | Burkholderia cepacia |
5.3.1.8 | expression of the His-tagged enzyme in Escherichia coli | Burkholderia cepacia |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.13 | EDTA | no activity is detected when 2 mM EDTA is added to the dialysis buffer | Burkholderia cepacia | |
5.3.1.8 | EDTA | no activity is detected when 2 mM EDTA is added to the dialysis buffer | Burkholderia cepacia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.13 | 2.9 | - |
GDP-mannose | pH 7.6, temperature not specified in the publication | Burkholderia cepacia | |
2.7.7.13 | 2.9 | - |
GDP-alpha-D-mannose | pH 7.6, 10 mM Mn2+, temperature not specified in the publication | Burkholderia cepacia | |
5.3.1.8 | 9.01 | - |
D-mannose 6-phosphate | pH 7.6, 5 mM Mg2+, temperature not specified in the publication | Burkholderia cepacia |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.13 | Ca2+ | GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ | Burkholderia cepacia | |
2.7.7.13 | Ca2+ | GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ | Burkholderia cepacia | |
2.7.7.13 | Mn2+ | GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ | Burkholderia cepacia | |
2.7.7.13 | Mn2+ | GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ | Burkholderia cepacia | |
5.3.1.8 | Ca2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
5.3.1.8 | Co2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
5.3.1.8 | Mg2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
5.3.1.8 | Mn2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
5.3.1.8 | Ni2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.13 | 55000 | - |
SDS-PAGE | Burkholderia cepacia |
2.7.7.13 | 55300 | - |
calculated from sequence | Burkholderia cepacia |
5.3.1.8 | 55000 | - |
SDS-PAGE | Burkholderia cepacia |
5.3.1.8 | 55300 | - |
calculated from sequence | Burkholderia cepacia |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.13 | Burkholderia cepacia | Q06XM8 | - |
- |
2.7.7.13 | Burkholderia cepacia IST408 | Q06XM8 | - |
- |
5.3.1.8 | Burkholderia cepacia | Q06XM8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.13 | - |
Burkholderia cepacia |
5.3.1.8 | - |
Burkholderia cepacia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.13 | diphosphate + GDP-alpha-D-mannose | - |
Burkholderia cepacia | GTP + alpha-D-mannose 1-phosphate | - |
? | |
2.7.7.13 | diphosphate + GDP-alpha-D-mannose | - |
Burkholderia cepacia IST408 | GTP + alpha-D-mannose 1-phosphate | - |
? | |
2.7.7.13 | diphosphate + GDP-mannose | - |
Burkholderia cepacia | GTP + alpha-D-mannose 1-phosphate | - |
? | |
2.7.7.13 | diphosphate + GDP-mannose | - |
Burkholderia cepacia IST408 | GTP + alpha-D-mannose 1-phosphate | - |
? | |
5.3.1.8 | D-Mannose 6-phosphate | - |
Burkholderia cepacia | D-Fructose 6-phosphate | - |
? | |
5.3.1.8 | D-Mannose 6-phosphate | - |
Burkholderia cepacia IST408 | D-Fructose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.13 | ? | x * 55000, SDS-PAGE | Burkholderia cepacia |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.13 | BceA | - |
Burkholderia cepacia |
2.7.7.13 | GDP-mannose pyrophosphorylase | - |
Burkholderia cepacia |
2.7.7.13 | More | bifunctional protein (type II PMI family) with phosphomannose isomerase (PMI, EC 5.3.1.8) and GDP-mannose pyrophosphorylase (GMP, EC 2.7.7.13) activities | Burkholderia cepacia |
5.3.1.8 | BceA | - |
Burkholderia cepacia |
5.3.1.8 | Phosphomannose isomerase | - |
Burkholderia cepacia |
5.3.1.8 | PMI | - |
Burkholderia cepacia |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.13 | 195.9 | - |
GDP-alpha-D-mannose | pH 7.6, 10 mM Mn2+, temperature not specified in the publication | Burkholderia cepacia | |
5.3.1.8 | 19.4 | - |
D-mannose 6-phosphate | pH 7.6, 5 mM Mg2+, temperature not specified in the publication | Burkholderia cepacia |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.13 | 7.6 | - |
assay at | Burkholderia cepacia |
5.3.1.8 | 7.6 | - |
assay at | Burkholderia cepacia |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.7.13 | Burkholderia cepacia | calculated from sequence | - |
6.03 |
5.3.1.8 | Burkholderia cepacia | calculated from sequence | - |
6.03 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.13 | malfunction | the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation | Burkholderia cepacia |
2.7.7.13 | metabolism | the bceA gene is part of the exopolysaccharide biosynthetic cluster | Burkholderia cepacia |
2.7.7.13 | physiological function | the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed | Burkholderia cepacia |
5.3.1.8 | malfunction | the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation | Burkholderia cepacia |
5.3.1.8 | physiological function | the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed | Burkholderia cepacia |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.13 | 195.9 | - |
GDP-mannose | pH 7.6, temperature not specified in the publication | Burkholderia cepacia |