EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.11 | expression in Escherichia coli | Thermus thermophilus |
3.1.26.11 | expression of the histidine-tagged enzyme in Escherichia coli | Thermotoga maritima |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.11 | Mg2+ | Bacillus subtilis pre-tRNACys is cleaved in the presence of Mn2+ but not in the presence of Mg2+ | Bacillus subtilis | |
3.1.26.11 | Mn2+ | Bacillus subtilis pre-tRNACys is cleaved in the presence of Mn2+ but not in the presence of Mg2+ | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.11 | Bacillus subtilis | P54548 | - |
- |
3.1.26.11 | Bacillus subtilis 168 | P54548 | - |
- |
3.1.26.11 | Pyrobaculum aerophilum | Q8ZTJ7 | - |
- |
3.1.26.11 | Pyrobaculum aerophilum DSM 7523 | Q8ZTJ7 | - |
- |
3.1.26.11 | Thermoplasma acidophilum | Q9HJ19 | - |
- |
3.1.26.11 | Thermotoga maritima | Q9WY50 | - |
- |
3.1.26.11 | Thermotoga maritima DSM 3109 | Q9WY50 | - |
- |
3.1.26.11 | Thermus thermophilus | Q53W74 | - |
- |
3.1.26.11 | Thermus thermophilus DSM 579 | Q53W74 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.11 | Bacillus subtilis pre-tRNATrp + H2O | cleaved well after C75 relatively | Thermus thermophilus | ? | - |
? | |
3.1.26.11 | Bacillus subtilis pre-tRNATrp + H2O | cleaved well after C75 relatively | Thermus thermophilus DSM 579 | ? | - |
? | |
3.1.26.11 | Bacillus subtilis tRNACys + H2O | efficient cleavage | Bacillus subtilis | ? | - |
? | |
3.1.26.11 | Bacillus subtilis tRNACys + H2O | efficient cleavage | Bacillus subtilis 168 | ? | - |
? | |
3.1.26.11 | Escherichia coli pre-tRNAPhe + H2O | cleavage is barely detected | Thermus thermophilus | ? | - |
? | |
3.1.26.11 | Escherichia coli pre-tRNAPhe + H2O | cleavage is barely detected | Thermus thermophilus DSM 579 | ? | - |
? | |
3.1.26.11 | human pre-tRNAArg + H2O | efficient cleavage | Bacillus subtilis | ? | - |
? | |
3.1.26.11 | human pre-tRNAArg + H2O | efficient cleavage | Thermoplasma acidophilum | ? | - |
? | |
3.1.26.11 | human pre-tRNAArg + H2O | efficient cleavage | Bacillus subtilis 168 | ? | - |
? | |
3.1.26.11 | Thermoplasma acidophilum pre-tRNAPhe + H2O | weak cleavage | Thermoplasma acidophilum | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | cleavage is barely detected | Thermus thermophilus | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75 | Thermotoga maritima | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | weak cleavage | Pyrobaculum aerophilum | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | weak cleavage | Pyrobaculum aerophilum DSM 7523 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75 | Thermotoga maritima DSM 3109 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(CCA) + H2O | cleavage is barely detected | Thermus thermophilus DSM 579 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(GUG) + H2O | efficient cleavage | Bacillus subtilis | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(GUG) + H2O | cleaved very inefficiently | Thermotoga maritima | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(GUG) + H2O | weak cleavage | Thermoplasma acidophilum | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(GUG) + H2O | efficient cleavage | Bacillus subtilis 168 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAArg(GUG) + H2O | cleaved very inefficiently | Thermotoga maritima DSM 3109 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAMet(CCA) + H2O | cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively | Thermotoga maritima | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAMet(CCA) + H2O | cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively | Thermotoga maritima DSM 3109 | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAMet(UAG) + H2O | cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively | Thermotoga maritima | ? | - |
? | |
3.1.26.11 | Thermotoga maritima pre-tRNAMet(UAG) + H2O | cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively | Thermotoga maritima DSM 3109 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.11 | dimer | gel filtration analysis shows that, only 20% of tRNase Z2 molecules form dimers, while tRNase Z molecules exist as dimers. This inefficient tRNase Z2 dimer formation may be attributed to the lack of alpha1, alpha2, and alpha3 that are important for dimerization of tRNase Z | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.11 | ribonuclease Z | - |
Thermoplasma acidophilum |
3.1.26.11 | ribonuclease Z | - |
Pyrobaculum aerophilum |
3.1.26.11 | TM0207 | - |
Thermotoga maritima |
3.1.26.11 | tRNase Z2 | - |
Thermotoga maritima |
3.1.26.11 | YP_145327 | - |
Thermus thermophilus |