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Literature summary extracted from

  • Yang, C.; Zhang, L.; Han, Q.; Liao, C.; Lan, J.; Ding, H.; Zhou, H.; Diao, X.; Li, J.
    Kynurenine aminotransferase 3/glutamine transaminase L/cysteine conjugate beta-lyase 2 is a major glutamine transaminase in the mouse kidney (2016), Biochem. Biophys. Rep., 8, 234-241 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.1.7 analysis the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus
2.6.1.15 analysis the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR Mus musculus
2.6.1.15 recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.15 methionine 57% inhibition at 5 mM, competitive inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.6
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus
2.6.1.15 0.7
-
 L-glutamine recombinant enzyme, pH 9.0, 38°C Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.15 L-glutamine + phenylpyruvate Mus musculus
-
 2-oxoglutaramate + L-phenylalanine
-
 ?
2.6.1.15 selenomethionine + phenylpyruvate Mus musculus
-
 2-oxo-gamma-methylselenobutyrate + L-phenylalanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Mus musculus
-
-
-
2.6.1.15 Mus musculus Q71RI9
-
-
4.4.1.13 Mus musculus Q71RI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration Mus musculus
2.6.1.15 recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 kidney
-
 Mus musculus
-
2.6.1.7 liver
-
 Mus musculus
-
2.6.1.7 additional information tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver Mus musculus
-
2.6.1.15 kidney
-
 Mus musculus
-
2.6.1.15 liver
-
 Mus musculus
-
2.6.1.15 additional information tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver Mus musculus
-
4.4.1.13 kidney much more abundant in the kidney than in the liver Mus musculus
-
4.4.1.13 liver much more abundant in the kidney than in the liver Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxobutyrate high activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxocaproate high activity, 2-oxocaproate is the best cosubstrate Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxoglutarate very low activity with 2-oxogltarate Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
 ?
2.6.1.7 L-kynurenine + 2-oxoleucine moderate activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxovalerate high activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxovaline low activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + glyoxylate high activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + hydroxyphenylpyruvate moderate activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + indol-3-pyruvate moderate activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + mercaptopyruvate moderate activity Mus musculus ?
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate moderate activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-aspartate
-
 ?
2.6.1.7 L-kynurenine + phenylpyruvate high activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine
-
 ?
2.6.1.7 L-kynurenine + pyruvate moderate activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 ?
2.6.1.7 additional information cosubstrate specificity, overview. No or poor activity with 2-oxoadipate and 2-oxoisoleucine. Recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK Mus musculus ?
-
-
2.6.1.15 L-glutamine + indol-3-pyruvate
-
 Mus musculus 2-oxoglutaramate + ?
-
 ?
2.6.1.15 L-glutamine + oxaloacetate
-
 Mus musculus 2-oxoglutaramate + L-aspartate
-
 ?
2.6.1.15 L-glutamine + phenylpyruvate
-
 Mus musculus 2-oxoglutaramate + L-phenylalanine
-
 ?
2.6.1.15 additional information recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK Mus musculus ?
-
-
2.6.1.15 selenomethionine + phenylpyruvate
-
 Mus musculus 2-oxo-gamma-methylselenobutyrate + L-phenylalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 KAT3
-
 Mus musculus
2.6.1.7 KAT3/GTL/CCBL2
-
 Mus musculus
2.6.1.7 kynurenine aminotransferase 3
-
 Mus musculus
2.6.1.7 More see also EC 2.6.1.64 and EC 4.4.1.13 Mus musculus
2.6.1.15 glutamine transaminase
-
 Mus musculus
2.6.1.15 glutamine transaminase L
-
 Mus musculus
2.6.1.15 GTL
-
 Mus musculus
2.6.1.15 KAT3/GTL/CCBL2
-
 Mus musculus
2.6.1.15 More see also EC 2.6.1.7 and EC 4.4.1.13 Mus musculus
4.4.1.13 KYAT3
-
 Mus musculus
4.4.1.13 kynurenine-oxoglutarate transaminase 3
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 38
-
 assay at Mus musculus
2.6.1.15 38
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.7 3.17
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus
2.6.1.15 2.67
-
 L-glutamine recombinant enzyme, pH 9.0, 38°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 7.5
-
 assay at Mus musculus
2.6.1.15 9
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate
-
 Mus musculus
2.6.1.15 pyridoxal 5'-phosphate
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.6.1.7 evolution kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) Mus musculus
2.6.1.7 additional information the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus
2.6.1.7 physiological function kynurenine aminotransferase 3 (KAT3) catalyzes the transamination of kynurenine to kynurenic acid Mus musculus
2.6.1.15 evolution kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) Mus musculus
2.6.1.15 additional information the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus
4.4.1.13 physiological function enzyme catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 and glutamine transaminase L. It is more efficient in transamination of glutamine with indo-3-pyruvate or oxaloacetate as aminogroup acceptor than the mouse KAT1 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.7 5.28
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus
2.6.1.15 3.81
-
 L-glutamine recombinant enzyme, pH 9.0, 38°C Mus musculus