EC Number | Application | Comment | Organism |
---|---|---|---|
2.6.1.7 | analysis | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
2.6.1.15 | analysis | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.7 | recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR | Mus musculus |
2.6.1.15 | recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.15 | methionine | 57% inhibition at 5 mM, competitive inhibition | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.7 | 0.6 | - |
phenylpyruvate | recombinant enzyme, pH 7.5, 38°C | Mus musculus | |
2.6.1.15 | 0.7 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.15 | L-glutamine + phenylpyruvate | Mus musculus | - |
2-oxoglutaramate + L-phenylalanine | - |
? | |
2.6.1.15 | selenomethionine + phenylpyruvate | Mus musculus | - |
2-oxo-gamma-methylselenobutyrate + L-phenylalanine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.7 | Mus musculus | - |
- |
- |
2.6.1.15 | Mus musculus | Q71RI9 | - |
- |
4.4.1.13 | Mus musculus | Q71RI9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.7 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration | Mus musculus |
2.6.1.15 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.7 | kidney | - |
Mus musculus | - |
2.6.1.7 | liver | - |
Mus musculus | - |
2.6.1.7 | additional information | tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver | Mus musculus | - |
2.6.1.15 | kidney | - |
Mus musculus | - |
2.6.1.15 | liver | - |
Mus musculus | - |
2.6.1.15 | additional information | tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver | Mus musculus | - |
4.4.1.13 | kidney | much more abundant in the kidney than in the liver | Mus musculus | - |
4.4.1.13 | liver | much more abundant in the kidney than in the liver | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.7 | L-kynurenine + 2-oxobutyrate | high activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + 2-oxocaproate | high activity, 2-oxocaproate is the best cosubstrate | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + 2-oxoglutarate | very low activity with 2-oxogltarate | Mus musculus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate | - |
? | |
2.6.1.7 | L-kynurenine + 2-oxoleucine | moderate activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + 2-oxovalerate | high activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + 2-oxovaline | low activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + glyoxylate | high activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + hydroxyphenylpyruvate | moderate activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + indol-3-pyruvate | moderate activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + mercaptopyruvate | moderate activity | Mus musculus | ? | - |
? | |
2.6.1.7 | L-kynurenine + oxaloacetate | moderate activity | Mus musculus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-aspartate | - |
? | |
2.6.1.7 | L-kynurenine + phenylpyruvate | high activity | Mus musculus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine | - |
? | |
2.6.1.7 | L-kynurenine + pyruvate | moderate activity | Mus musculus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
? | |
2.6.1.7 | additional information | cosubstrate specificity, overview. No or poor activity with 2-oxoadipate and 2-oxoisoleucine. Recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK | Mus musculus | ? | - |
- |
|
2.6.1.15 | L-glutamine + indol-3-pyruvate | - |
Mus musculus | 2-oxoglutaramate + ? | - |
? | |
2.6.1.15 | L-glutamine + oxaloacetate | - |
Mus musculus | 2-oxoglutaramate + L-aspartate | - |
? | |
2.6.1.15 | L-glutamine + phenylpyruvate | - |
Mus musculus | 2-oxoglutaramate + L-phenylalanine | - |
? | |
2.6.1.15 | additional information | recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK | Mus musculus | ? | - |
- |
|
2.6.1.15 | selenomethionine + phenylpyruvate | - |
Mus musculus | 2-oxo-gamma-methylselenobutyrate + L-phenylalanine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.7 | KAT3 | - |
Mus musculus |
2.6.1.7 | KAT3/GTL/CCBL2 | - |
Mus musculus |
2.6.1.7 | kynurenine aminotransferase 3 | - |
Mus musculus |
2.6.1.7 | More | see also EC 2.6.1.64 and EC 4.4.1.13 | Mus musculus |
2.6.1.15 | glutamine transaminase | - |
Mus musculus |
2.6.1.15 | glutamine transaminase L | - |
Mus musculus |
2.6.1.15 | GTL | - |
Mus musculus |
2.6.1.15 | KAT3/GTL/CCBL2 | - |
Mus musculus |
2.6.1.15 | More | see also EC 2.6.1.7 and EC 4.4.1.13 | Mus musculus |
4.4.1.13 | KYAT3 | - |
Mus musculus |
4.4.1.13 | kynurenine-oxoglutarate transaminase 3 | - |
Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.7 | 38 | - |
assay at | Mus musculus |
2.6.1.15 | 38 | - |
assay at | Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.7 | 3.17 | - |
phenylpyruvate | recombinant enzyme, pH 7.5, 38°C | Mus musculus | |
2.6.1.15 | 2.67 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.7 | 7.5 | - |
assay at | Mus musculus |
2.6.1.15 | 9 | - |
assay at | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.7 | pyridoxal 5'-phosphate | - |
Mus musculus | |
2.6.1.15 | pyridoxal 5'-phosphate | - |
Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.7 | evolution | kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) | Mus musculus |
2.6.1.7 | additional information | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
2.6.1.7 | physiological function | kynurenine aminotransferase 3 (KAT3) catalyzes the transamination of kynurenine to kynurenic acid | Mus musculus |
2.6.1.15 | evolution | kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) | Mus musculus |
2.6.1.15 | additional information | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
4.4.1.13 | physiological function | enzyme catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 and glutamine transaminase L. It is more efficient in transamination of glutamine with indo-3-pyruvate or oxaloacetate as aminogroup acceptor than the mouse KAT1 | Mus musculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.7 | 5.28 | - |
phenylpyruvate | recombinant enzyme, pH 7.5, 38°C | Mus musculus | |
2.6.1.15 | 3.81 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus |