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Literature summary extracted from

  • Xu, J.; Tian, Y.; Peng, R.; Zhu, B.; Gao, J.; Yao, Q.
    Characterization of a thermostable beta-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana (2012), Appl. Microbiol. Biotechnol., 95, 1211-1219 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.31 butanol 1%, 1.5fold activation Thermotoga maritima
3.2.1.31 CTAB 1%, 1.3fold activation Thermotoga maritima
3.2.1.31 Dimethylsulfoxide 1%, 1.3fold activation Thermotoga maritima
3.2.1.31 EDTA 1%, 1.25fold activation Thermotoga maritima
3.2.1.31 methanol 1%, 1.4fold activation Thermotoga maritima
3.2.1.31 Triton X-100 1%, 1.9fold activation Thermotoga maritima
3.2.1.31 Tween-20 1%, 1.7fold activation Thermotoga maritima

Application

EC Number Application Comment Organism
3.2.1.31 molecular biology use of beta-glucuronidase from Thermotoga maritima as a thermostable marker in higher plants, reporter enzyme in plant genetic research Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.31 expression in both Escherichia coli and Arabidopsis thaliana. The enzyme expressed in transformed Escherichia coli cells exhibits maximum hydrolytic activity at 65°C and pH 6.5 and retains more than 80% activity after incubation at 85°C for 30 min. Activity in transgenic Arabidopsis thaliana plants containing TmGUSI is also stable over the temperature range 65-80°C Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 Al3+ 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 Ca2+ recombinant enzyme expressed in Escherichia coli is slightly inhibited Thermotoga maritima
3.2.1.31 Citric acid 1%, 42% inhibition Thermotoga maritima
3.2.1.31 Cr3+ 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 Cu2+ 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 dithiothreitol 1%, 22% inhibition Thermotoga maritima
3.2.1.31 Fe2+ 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 Fe3+ 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 Mn2+ 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 Pb2+ 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima
3.2.1.31 SDS 0.1% v/v, complete inhibition Thermotoga maritima
3.2.1.31 Urea 1%, 38% inhibition Thermotoga maritima
3.2.1.31 Zn2+ 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.31 K+ below 0.1 mM, recombinant enzyme expressed in Escherichia coli is activated Thermotoga maritima
3.2.1.31 Mg2+ below 1 mM, recombinant enzyme expressed in Escherichia coli is activated Thermotoga maritima
3.2.1.31 Na+ below 1 mM, recombinant enzyme expressed in Escherichia coli is activated Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Thermotoga maritima DQ459484
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O
-
 Thermotoga maritima 4-methylumbelliferone + D-glucuronic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 ? x * 65672, calculated from sequence Thermotoga maritima
3.2.1.31 ? x * 66000, enzyme expressed in Escherichia coli, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 TmGUSI
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 65
-
 enzyme expressed in Escherichia coli Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 65 80 30 min, enzyme expressed in Arabidopsis thaliana is stable Thermotoga maritima
3.2.1.31 85
-
 30 min, enzyme expressed in Escherichia coli retains more than 80% of its activity Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 6.5
-
 enzyme expressed in Escherichia coli Thermotoga maritima