EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.85 | - |
Rhizobacter gummiphilus |
1.13.11.87 | expressed in a DELTAroxA35Y/DELTAroxB35Y background of Steroidobacter cummioxidans 35Y | Rhizobacter gummiphilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.85 | 71500 | - |
mature protein, SDS-PAGE | Rhizobacter gummiphilus |
1.13.11.85 | 74400 | - |
preprotein, SDS-PAGE | Rhizobacter gummiphilus |
1.13.11.87 | 70800 | - |
mature protein, SDS-PAGE | Rhizobacter gummiphilus |
1.13.11.87 | 74000 | - |
preprotein, SDS-PAGE | Rhizobacter gummiphilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.85 | cis-1,4-polyisoprene + O2 | Rhizobacter gummiphilus | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.85 | cis-1,4-polyisoprene + O2 | Rhizobacter gummiphilus NS21 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | Rhizobacter gummiphilus | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | ? | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | Rhizobacter gummiphilus NS21 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.85 | Rhizobacter gummiphilus | - |
- |
- |
1.13.11.85 | Rhizobacter gummiphilus NS21 | - |
- |
- |
1.13.11.87 | Rhizobacter gummiphilus | - |
- |
- |
1.13.11.87 | Rhizobacter gummiphilus NS21 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.85 | - |
Rhizobacter gummiphilus |
1.13.11.87 | - |
Rhizobacter gummiphilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.85 | 0.1 | - |
23°C, pH not specified in the publication | Rhizobacter gummiphilus |
1.13.11.87 | 4.8 | - |
23°C, pH not specified in the publication | Rhizobacter gummiphilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.85 | cis-1,4-polyisoprene + O2 | - |
Rhizobacter gummiphilus | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.85 | cis-1,4-polyisoprene + O2 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.85 | cis-1,4-polyisoprene + O2 | - |
Rhizobacter gummiphilus NS21 | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.85 | cis-1,4-polyisoprene + O2 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus NS21 | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | - |
Rhizobacter gummiphilus | ? | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus | ? | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | - |
Rhizobacter gummiphilus NS21 | ? | - |
? | |
1.13.11.87 | cis-1,4-polyisoprene + O2 | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus NS21 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.85 | ? | x * 71500, mature protein, SDS-PAGE | Rhizobacter gummiphilus |
1.13.11.87 | ? | x * 70800, mature protein, SDS-PAGE | Rhizobacter gummiphilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.85 | roxA | - |
Rhizobacter gummiphilus |
1.13.11.87 | RoxB | - |
Rhizobacter gummiphilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.85 | malfunction | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus |
1.13.11.87 | malfunction | Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | Rhizobacter gummiphilus |