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Literature summary extracted from

  • Chen, M.; Huang, X.; Zhong, C.; Li, J.; Lu, X.
    Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus (2016), Appl. Microbiol. Biotechnol., 100, 7541-7548 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.25 expressed in Escherichia coli BL21(DE3) cells Aspergillus terreus
4.1.3.25 expression in Escherichia coli BL21 (DE3) Aspergillus terreus
4.1.3.46 expression in Escherichia coli BL21 (DE3) Aspergillus terreus
4.2.1.56 expression in Escherichia coli BL21 (DE3) Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.25 (3S)-citramalyl-CoA Aspergillus terreus
-
 acetyl-CoA + pyruvate
-
 ?
4.1.3.25 (3S)-citramalyl-CoA Aspergillus terreus LYT10
-
 acetyl-CoA + pyruvate
-
 ?
4.1.3.25 citramalyl-CoA Aspergillus terreus
-
 acetyl-CoA + pyruvate
-
 ?
4.1.3.25 citramalyl-CoA Aspergillus terreus LYT10
-
 acetyl-CoA + pyruvate
-
 ?
4.1.3.46 citramalyl-CoA Aspergillus terreus
-
 acetyl-CoA + pyruvate
-
 ?
4.1.3.46 citramalyl-CoA Aspergillus terreus LYT10
-
 acetyl-CoA + pyruvate
-
 ?
4.2.1.56 Itaconyl-CoA + H2O Aspergillus terreus the enzyme is involved in the catabolic pathway of itaconic acid. Although Aspergillus terreus LYT10 can catabolize itaconic acid, it cannot grow in the medium using itaconic acid (with concentrations from 5 to 40 g/l) as the sole carbon source Citramalyl-CoA
-
 ?
4.2.1.56 Itaconyl-CoA + H2O Aspergillus terreus LYT10 the enzyme is involved in the catabolic pathway of itaconic acid. Although Aspergillus terreus LYT10 can catabolize itaconic acid, it cannot grow in the medium using itaconic acid (with concentrations from 5 to 40 g/l) as the sole carbon source Citramalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.25 Aspergillus terreus A0A172MLA1
-
-
4.1.3.25 Aspergillus terreus LYT10 A0A172MLA1
-
-
4.1.3.46 Aspergillus terreus A0A172MLA1
-
-
4.1.3.46 Aspergillus terreus LYT10 A0A172MLA1
-
-
4.2.1.56 Aspergillus terreus A0A172ML99
-
-
4.2.1.56 Aspergillus terreus LYT10 A0A172ML99
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.25
-
 Aspergillus terreus
4.1.3.25 nickel chelating resin column chromatography Aspergillus terreus
4.1.3.46
-
 Aspergillus terreus
4.2.1.56
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.25 (3S)-citramalyl-CoA
-
 Aspergillus terreus acetyl-CoA + pyruvate
-
 ?
4.1.3.25 (3S)-citramalyl-CoA
-
 Aspergillus terreus LYT10 acetyl-CoA + pyruvate
-
 ?
4.1.3.25 citramalyl-CoA
-
 Aspergillus terreus acetyl-CoA + pyruvate
-
 ?
4.1.3.25 citramalyl-CoA
-
 Aspergillus terreus LYT10 acetyl-CoA + pyruvate
-
 ?
4.1.3.46 citramalyl-CoA
-
 Aspergillus terreus acetyl-CoA + pyruvate
-
 ?
4.1.3.46 citramalyl-CoA
-
 Aspergillus terreus LYT10 acetyl-CoA + pyruvate
-
 ?
4.2.1.56 Itaconyl-CoA + H2O
-
 Aspergillus terreus Citramalyl-CoA
-
 ?
4.2.1.56 Itaconyl-CoA + H2O the enzyme is involved in the catabolic pathway of itaconic acid. Although Aspergillus terreus LYT10 can catabolize itaconic acid, it cannot grow in the medium using itaconic acid (with concentrations from 5 to 40 g/l) as the sole carbon source Aspergillus terreus Citramalyl-CoA
-
 ?
4.2.1.56 Itaconyl-CoA + H2O
-
 Aspergillus terreus LYT10 Citramalyl-CoA
-
 ?
4.2.1.56 Itaconyl-CoA + H2O the enzyme is involved in the catabolic pathway of itaconic acid. Although Aspergillus terreus LYT10 can catabolize itaconic acid, it cannot grow in the medium using itaconic acid (with concentrations from 5 to 40 g/l) as the sole carbon source Aspergillus terreus LYT10 Citramalyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.25 CclA
-
 Aspergillus terreus
4.1.3.25 citramalyl-CoA lyase
-
 Aspergillus terreus
4.1.3.46 CclA
-
 Aspergillus terreus
4.1.3.46 citramalyl-CoA lyase
-
 Aspergillus terreus
4.2.1.56 IchA
-
 Aspergillus terreus

Expression

EC Number Organism Comment Expression
4.1.3.25 Aspergillus terreus upregulated at both transcription and protein levels When 40 g/l of exogenous itaconic acid is added into the medium at the beginning of fermentation up
4.1.3.46 Aspergillus terreus upregulated at both transcription and protein levels When 40 g/l of exogenous itaconic acid is added into the medium at the beginning of fermentation up
4.2.1.56 Aspergillus terreus upregulated at both transcription and protein levels, when 40 g/l of exogenous itaconic acid is added into the medium at the beginning of fermentation up

General Information

EC Number General Information Comment Organism
4.1.3.25 metabolism the enzyme is involved in the catabolic pathway of itaconic acid Aspergillus terreus
4.1.3.25 metabolism the enzyme is involved in the itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus Aspergillus terreus
4.1.3.46 metabolism the enzyme is involved in the catabolic pathway of itaconic acid Aspergillus terreus
4.2.1.56 metabolism the enzyme is involved in the catabolic pathway of itaconic acid Aspergillus terreus