EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.3.114 | synthesis | recombinant expression in Aspergillus nidulans resulting in production of gamma-terpinene. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds, e.g. gamma-terpinene | Citrus unshiu |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.30 | gene AN1594, the AN1594 overexpression strain A772 oe:AN1594 is created by placing AN1594 under the control of the constitutively active gpdA promoter in Aspergillus nidulans strain A772, quantitative RT-PCR enzyme expression analysis | Aspergillus nidulans |
4.2.3.114 | gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds | Citrus unshiu |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.30 | Mg2+ | required | Aspergillus nidulans | |
4.2.3.114 | Mg2+ | required | Citrus unshiu |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.30 | ent-copalyl diphosphate | Aspergillus nidulans | - |
ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.30 | ent-copalyl diphosphate | Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | - |
ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.30 | ent-copalyl diphosphate | Aspergillus nidulans A4 | - |
ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.114 | geranyl diphosphate | Citrus unshiu | - |
gamma-terpinene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.30 | Aspergillus nidulans | Q5BCY6 | - |
- |
4.2.3.30 | Aspergillus nidulans | Q5BCY6 | i.e. Emericella nidulans | - |
4.2.3.30 | Aspergillus nidulans A4 | Q5BCY6 | - |
- |
4.2.3.30 | Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | Q5BCY6 | i.e. Emericella nidulans | - |
4.2.3.114 | Citrus unshiu | Q6F5H0 | i.e. Citrus nobilis var. unshiu | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.30 | leaf | - |
Aspergillus nidulans | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.30 | ent-copalyl diphosphate | - |
Aspergillus nidulans | ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.30 | ent-copalyl diphosphate | - |
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.30 | ent-copalyl diphosphate | - |
Aspergillus nidulans A4 | ent-pimara-8(14),15-diene + diphosphate | - |
? | |
4.2.3.30 | additional information | GC/MS analysis of enzyme products | Aspergillus nidulans | ? | - |
? | |
4.2.3.30 | additional information | GC/MS analysis of enzyme products | Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | ? | - |
? | |
4.2.3.30 | additional information | GC/MS analysis of enzyme products | Aspergillus nidulans A4 | ? | - |
? | |
4.2.3.114 | geranyl diphosphate | - |
Citrus unshiu | gamma-terpinene + diphosphate | - |
? | |
4.2.3.114 | additional information | product identification and quantification by GC-MS analysis | Citrus unshiu | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.30 | AN1594 | locus name | Aspergillus nidulans |
4.2.3.30 | ANIA_01594 | gene name, UniProt | Aspergillus nidulans |
4.2.3.30 | ent-pimara-8(14),15-diene synthase | - |
Aspergillus nidulans |
4.2.3.30 | pbcA | - |
Aspergillus nidulans |
4.2.3.30 | PD synthase | - |
Aspergillus nidulans |
4.2.3.114 | CitMTSL61 | - |
Citrus unshiu |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.114 | 30 | - |
assay at | Citrus unshiu |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.114 | 7.6 | - |
assay at | Citrus unshiu |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.30 | Aspergillus nidulans | wild-type Aspergillus nidulans strain overexpressing the cluster-specific transcription factor, pbcR, shows upregulation of the cluster genes leading to ent-pimara-8(14),15-diene production in Aspergillus nidulans and 11.7fold increased expression of gene ANIA_01594 | up |