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Literature summary extracted from

  • Bromann, K.; Toivari, M.; Viljanen, K.; Ruohonen, L.; Nakari-Setaelae, T.
    Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-terpinene production (2016), Appl. Microbiol. Biotechnol., 100, 6345-6359 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.114 synthesis recombinant expression in Aspergillus nidulans resulting in production of gamma-terpinene. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds, e.g. gamma-terpinene Citrus unshiu

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.30 gene AN1594, the AN1594 overexpression strain A772 oe:AN1594 is created by placing AN1594 under the control of the constitutively active gpdA promoter in Aspergillus nidulans strain A772, quantitative RT-PCR enzyme expression analysis Aspergillus nidulans
4.2.3.114 gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds Citrus unshiu

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.30 Mg2+ required Aspergillus nidulans
4.2.3.114 Mg2+ required Citrus unshiu

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.30 ent-copalyl diphosphate Aspergillus nidulans
-
ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.30 ent-copalyl diphosphate Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139
-
ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.30 ent-copalyl diphosphate Aspergillus nidulans A4
-
ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.114 geranyl diphosphate Citrus unshiu
-
gamma-terpinene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.30 Aspergillus nidulans Q5BCY6
-
-
4.2.3.30 Aspergillus nidulans Q5BCY6 i.e. Emericella nidulans
-
4.2.3.30 Aspergillus nidulans A4 Q5BCY6
-
-
4.2.3.30 Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 Q5BCY6 i.e. Emericella nidulans
-
4.2.3.114 Citrus unshiu Q6F5H0 i.e. Citrus nobilis var. unshiu
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.30 leaf
-
Aspergillus nidulans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.30 ent-copalyl diphosphate
-
Aspergillus nidulans ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.30 ent-copalyl diphosphate
-
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.30 ent-copalyl diphosphate
-
Aspergillus nidulans A4 ent-pimara-8(14),15-diene + diphosphate
-
?
4.2.3.30 additional information GC/MS analysis of enzyme products Aspergillus nidulans ?
-
?
4.2.3.30 additional information GC/MS analysis of enzyme products Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 ?
-
?
4.2.3.30 additional information GC/MS analysis of enzyme products Aspergillus nidulans A4 ?
-
?
4.2.3.114 geranyl diphosphate
-
Citrus unshiu gamma-terpinene + diphosphate
-
?
4.2.3.114 additional information product identification and quantification by GC-MS analysis Citrus unshiu ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.30 AN1594 locus name Aspergillus nidulans
4.2.3.30 ANIA_01594 gene name, UniProt Aspergillus nidulans
4.2.3.30 ent-pimara-8(14),15-diene synthase
-
Aspergillus nidulans
4.2.3.30 pbcA
-
Aspergillus nidulans
4.2.3.30 PD synthase
-
Aspergillus nidulans
4.2.3.114 CitMTSL61
-
Citrus unshiu

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.114 30
-
assay at Citrus unshiu

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.114 7.6
-
assay at Citrus unshiu

Expression

EC Number Organism Comment Expression
4.2.3.30 Aspergillus nidulans wild-type Aspergillus nidulans strain overexpressing the cluster-specific transcription factor, pbcR, shows upregulation of the cluster genes leading to ent-pimara-8(14),15-diene production in Aspergillus nidulans and 11.7fold increased expression of gene ANIA_01594 up