EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.85 | - |
Xanthomonas sp. 35Y |
1.13.11.87 | - |
Xanthomonas sp. 35Y |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.85 | CO | carbon monoxide strongly inhibits the polyisoprene cleavage reaction of RoxAXsp | Xanthomonas sp. 35Y | |
1.13.11.87 | CO | oxygen consumption by RoxB is inhibited when carbon monoxide is added to an ongoing polyisoprene cleavage reaction | Xanthomonas sp. 35Y | |
1.13.11.87 | diethyldithiocarbamate | - |
Xanthomonas sp. 35Y | |
1.13.11.87 | imidazole | binding of this compounds to the active heme site inhibits the enzyme | Xanthomonas sp. 35Y | |
1.13.11.87 | additional information | the use of 1 or 10 mM EDTA has no effect on the rubber-cleaving activity of RoxB | Xanthomonas sp. 35Y | |
1.13.11.87 | Pyridine | binding of this compounds to the active heme site inhibits the enzyme | Xanthomonas sp. 35Y | |
1.13.11.87 | SDS | - |
Xanthomonas sp. 35Y |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.85 | 71500 | - |
mature protein | Xanthomonas sp. 35Y |
1.13.11.85 | 74700 | - |
preprotein | Xanthomonas sp. 35Y |
1.13.11.87 | 70300 | - |
- |
Xanthomonas sp. 35Y |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.85 | Xanthomonas sp. 35Y | A0A1Y0DKQ1 | - |
- |
1.13.11.87 | Xanthomonas sp. 35Y | A0A1S6Q8F9 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.13.11.85 | proteolytic modification | the preprotein has a MW of 74700 Da, the mature protein has MW of 71500 Da | Xanthomonas sp. 35Y |
1.13.11.87 | proteolytic modification | the preprotein has a MW of 73800 Da, the mature protein has MW of 71500 Da | Xanthomonas sp. 35Y |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.85 | - |
Xanthomonas sp. 35Y |
1.13.11.87 | - |
Xanthomonas sp. 35Y |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.85 | 1.9 | - |
30°C, pH not specified in the publication | Xanthomonas sp. 35Y |
1.13.11.85 | 2.6 | - |
37°C, pH not specified in the publication | Xanthomonas sp. 35Y |
1.13.11.87 | 4.5 | - |
23°C, pH and temperature not specified in the publication | Xanthomonas sp. 35Y |
1.13.11.87 | 5.7 | - |
30°C, pH and temperature not specified in the publication | Xanthomonas sp. 35Y |
1.13.11.87 | 6.4 | - |
37°C, pH and temperature not specified in the publication | Xanthomonas sp. 35Y |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.85 | poly(cis-1,4-isoprene) + O2 | - |
Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
1.13.11.87 | poly(cis-1,4-isoprene) + O2 | the enzyme cleaves cis-1,4-polyisoprene polymers into a mixture of C20, C25, and higher oligo-isoprenoids with terminal aldehyde and keto groups were identified | Xanthomonas sp. 35Y | (4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal + (4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal + (4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.85 | RoxB | - |
Xanthomonas sp. 35Y |
1.13.11.87 | RoxB | - |
Xanthomonas sp. 35Y |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.87 | 6 | 8 | - |
Xanthomonas sp. 35Y |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.87 | 5 | 9 | pH 5.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity | Xanthomonas sp. 35Y |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.85 | heme | c-type diheme protein | Xanthomonas sp. 35Y | |
1.13.11.87 | heme | 2 c-type hemes | Xanthomonas sp. 35Y | |
1.13.11.87 | heme | 2 c-type hemes. Both heme sites of RoxB are present in an oxidized Fe3+ form | Xanthomonas sp. 35Y |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.13.11.85 | Xanthomonas sp. 35Y | expression of the roxA gene is upregulated during growth on polyisoprene | up |
1.13.11.87 | Xanthomonas sp. 35Y | expression of the roxA gene is upregulated during growth on polyisoprene | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.87 | metabolism | the synergistic effect of the simultaneous presence of RoxA35Y and RoxB35Y enables the organism to convert rubber extracellularly at high efficiency to just one cleavage product of defined length ((4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal) that can be taken up by only one transport protein and can be used as a source of carbon and energy | Xanthomonas sp. 35Y |