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Literature summary extracted from

  • Birke, J.; Roether, W.; Jendrossek, D.
    RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp) (2017), Appl. Environ. Microbiol., 83, e00721-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.85
-
Xanthomonas sp. 35Y
1.13.11.87
-
Xanthomonas sp. 35Y

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.85 CO carbon monoxide strongly inhibits the polyisoprene cleavage reaction of RoxAXsp Xanthomonas sp. 35Y
1.13.11.87 CO oxygen consumption by RoxB is inhibited when carbon monoxide is added to an ongoing polyisoprene cleavage reaction Xanthomonas sp. 35Y
1.13.11.87 diethyldithiocarbamate
-
Xanthomonas sp. 35Y
1.13.11.87 imidazole binding of this compounds to the active heme site inhibits the enzyme Xanthomonas sp. 35Y
1.13.11.87 additional information the use of 1 or 10 mM EDTA has no effect on the rubber-cleaving activity of RoxB Xanthomonas sp. 35Y
1.13.11.87 Pyridine binding of this compounds to the active heme site inhibits the enzyme Xanthomonas sp. 35Y
1.13.11.87 SDS
-
Xanthomonas sp. 35Y

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.85 71500
-
mature protein Xanthomonas sp. 35Y
1.13.11.85 74700
-
preprotein Xanthomonas sp. 35Y
1.13.11.87 70300
-
-
Xanthomonas sp. 35Y

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.85 Xanthomonas sp. 35Y A0A1Y0DKQ1
-
-
1.13.11.87 Xanthomonas sp. 35Y A0A1S6Q8F9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.85 proteolytic modification the preprotein has a MW of 74700 Da, the mature protein has MW of 71500 Da Xanthomonas sp. 35Y
1.13.11.87 proteolytic modification the preprotein has a MW of 73800 Da, the mature protein has MW of 71500 Da Xanthomonas sp. 35Y

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.85
-
Xanthomonas sp. 35Y
1.13.11.87
-
Xanthomonas sp. 35Y

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.85 1.9
-
30°C, pH not specified in the publication Xanthomonas sp. 35Y
1.13.11.85 2.6
-
37°C, pH not specified in the publication Xanthomonas sp. 35Y
1.13.11.87 4.5
-
23°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y
1.13.11.87 5.7
-
30°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y
1.13.11.87 6.4
-
37°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.85 poly(cis-1,4-isoprene) + O2
-
Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
?
1.13.11.87 poly(cis-1,4-isoprene) + O2 the enzyme cleaves cis-1,4-polyisoprene polymers into a mixture of C20, C25, and higher oligo-isoprenoids with terminal aldehyde and keto groups were identified Xanthomonas sp. 35Y (4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal + (4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal + (4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.85 RoxB
-
Xanthomonas sp. 35Y
1.13.11.87 RoxB
-
Xanthomonas sp. 35Y

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.87 6 8
-
Xanthomonas sp. 35Y

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.87 5 9 pH 5.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity Xanthomonas sp. 35Y

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.85 heme c-type diheme protein Xanthomonas sp. 35Y
1.13.11.87 heme 2 c-type hemes Xanthomonas sp. 35Y
1.13.11.87 heme 2 c-type hemes. Both heme sites of RoxB are present in an oxidized Fe3+ form Xanthomonas sp. 35Y

Expression

EC Number Organism Comment Expression
1.13.11.85 Xanthomonas sp. 35Y expression of the roxA gene is upregulated during growth on polyisoprene up
1.13.11.87 Xanthomonas sp. 35Y expression of the roxA gene is upregulated during growth on polyisoprene up

General Information

EC Number General Information Comment Organism
1.13.11.87 metabolism the synergistic effect of the simultaneous presence of RoxA35Y and RoxB35Y enables the organism to convert rubber extracellularly at high efficiency to just one cleavage product of defined length ((4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal) that can be taken up by only one transport protein and can be used as a source of carbon and energy Xanthomonas sp. 35Y