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Literature summary extracted from

  • Zang, Y.; Jiang, T.; Cong, Y.; Zheng, Z.; Ouyang, J.
    Molecular characterization of a recombinant Zea mays phenylalanine ammonia-lyase (ZmPAL2) and its application in trans-cinnamic acid production from L-phenylalanine (2015), Appl. Biochem. Biotechnol., 176, 924-937 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.24 synthesis the recombinant ZmPAL2 is a good candidate for the production of trans-cinnamic acid. The recombinant ZmPAL2 can effectively catalyze L-phenylalanine to trans-cinnamic acid, and the trans-cinnamic acid concentration can reach up to 5 g/l Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.24 expressed in Escherichia coli BL21 (DE3) Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 Co2+ 1 mM, 17% loss of activity Zea mays
4.3.1.24 Cu2+ 1 mM, 51% loss of activity Zea mays
4.3.1.24 K+ 1 mM, 1% loss of activity Zea mays
4.3.1.24 Mn2+ 1 mM, 21% loss of activity Zea mays
4.3.1.24 Na+ 1 mM, 3% loss of activity Zea mays
4.3.1.24 trans-cinnamate product inhibition, 1.4 mM, about 40% loss of activity Zea mays
4.3.1.24 Zn2+ 1 mM, 70% loss of activity Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 2.494
-
 L-phenylalanine pH 9.0, 55°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.24 Ba2+ 1 mM, weak enhancement of activity Zea mays
4.3.1.24 Ca2+ 1 mM, weak enhancement of activity Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 80000
-
 SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Zea mays C0HJ40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
 Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.24 7.14
-
 pH 9.0, 55°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-phenylalanine the recombinant ZmPAL2 exhibits a high phenylalanine ammonia-lyase activity (7.14 U/mg) and a weak tyrosine ammonia-lyase activity Zea mays trans-cinnamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 ? x * 80000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
4.3.1.24 ZmPAL2
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 55
-
-
 Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.24 35 70 35°C: about 50% of maximal activity, 70°C: about 75% of maximal activity Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.24 60
-
 6 h, about 50% loss of activity Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 9
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.24 7.5 10 pH 7.5: about 40% of maximal activity, pH 10.0: about 75% of maximal activity Zea mays

General Information

EC Number General Information Comment Organism
4.3.1.24 metabolism the enzyme has crucial role in secondary phenylpropanoid metabolism of plants Zea mays