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Literature summary extracted from

  • Frister, T.; Hartwig, S.; Alemdar, S.; Schnatz, K.; Thoens, L.; Scheper, T.; Beutel, S.
    Characterisation of a recombinant patchoulol synthase variant for biocatalytic production of terpenes (2015), Appl. Biochem. Biotechnol., 176, 2185-2201 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.70 expressed in Escherichia coli BL21(DE3) cells Pogostemon cablin

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.70 0.008
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.70 Mg2+ required Pogostemon cablin
4.2.3.70 additional information no activity with Ca2+, Ba2+, Mn2+, Ni2+, Co2+and Cu2+ Pogostemon cablin

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.70 (2E,6Z)-farnesyl diphosphate Pogostemon cablin
-
 patchoulol + diphosphate
-
 ?
4.2.3.70 (2Z,6E)-farnesyl diphosphate Pogostemon cablin
-
 patchoulol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.70 Pogostemon cablin Q49SP3 Indian Patchouli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.70 HisTrap column chromatography Pogostemon cablin

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.70 (2E,6Z)-farnesyl diphosphate
-
 Pogostemon cablin patchoulol + diphosphate
-
 ?
4.2.3.70 (2Z,6E)-farnesyl diphosphate
-
 Pogostemon cablin patchoulol + diphosphate
-
 ?
4.2.3.70 Geranyl diphosphate
-
 Pogostemon cablin ?
-
 ?
4.2.3.70 additional information no activity with geranylgeranyl diphosphate Pogostemon cablin ?
-
 ?
4.2.3.70 neryl diphosphate
-
 Pogostemon cablin ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.70 PTS variant 1 Pogostemon cablin

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.70 38
-
-
 Pogostemon cablin

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.70 0.072
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.70 7.3
-
-
 Pogostemon cablin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.70 9
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin