EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.1 | heterologous expression of the His6-tagged enzyme in Escherichia coli Rosetta (DE3) | Methylotuvimicrobium alcaliphilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 0.11 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
2.7.2.1 | 5.6 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | Co2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum | |
2.7.2.1 | Mg2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum | |
2.7.2.1 | Mn2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 43600 | - |
calculated from sequence | Methylotuvimicrobium alcaliphilum |
2.7.2.1 | 45000 | - |
recombinant His6-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
2.7.2.1 | 80000 | - |
native gradient electrophoresis | Methylotuvimicrobium alcaliphilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methylotuvimicrobium alcaliphilum | G4T0C7 | - |
- |
2.7.2.1 | Methylotuvimicrobium alcaliphilum 20Z | G4T0C7 | - |
- |
4.1.2.9 | Methylotuvimicrobium alcaliphilum | G4T4R3 | - |
- |
4.1.2.9 | Methylotuvimicrobium alcaliphilum 20Z | G4T4R3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.1 | - |
Methylotuvimicrobium alcaliphilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ADP + acetyl phosphate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum | ATP + acetate | - |
r | |
2.7.2.1 | ADP + acetyl phosphate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum 20Z | ATP + acetate | - |
r | |
2.7.2.1 | ATP + acetate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + acetate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.1 | homodimer | 2 * 43600, calculated from sequence | Methylotuvimicrobium alcaliphilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.1 | ACK | - |
Methylotuvimicrobium alcaliphilum |
4.1.2.9 | Xfp | - |
Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 70 | - |
- |
Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 30 | 70 | enzyme activity is 5fold higher at 70°C compared to that at 30°C | Methylotuvimicrobium alcaliphilum |
2.7.2.1 | 55 | 75 | 55°C: about 50% of maximal activity, 75°C: about 85% of maximal activity | Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 30 | 70 | 50-70% activity is retained after a 3 h exposure at 30-70°C | Methylotuvimicrobium alcaliphilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 233.3 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
2.7.2.1 | 316.7 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 8 | 8.5 | - |
Methylotuvimicrobium alcaliphilum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.2.9 | metabolism | the phosphoketolase route as a mechanism of acetyl-CoA synthesis provides an advantage to microbes whose carbon assimilation proceeds via de novo formation of the C-C bond. The draft analysis of the Methylomicrobium alcaliphilum genome reveals the open reading frames (ORFs) that encode a putative D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase (XFP, EC 4.1.2.9/EC 4.1.2.22) and acetate kinase (AcK, EC 2.7.2.1). The co-location of the xfp- and ack-like genes in the chromosome indicates that the phosphoketolase pathway in Methylomicrobium alcaliphilum 20Z can represent a pathway for phosphosugars breakdown and an additional source of C2 compounds for acetyl-CoA synthesis | Methylotuvimicrobium alcaliphilum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 2500 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
2.7.2.1 | 2879 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |