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Literature summary extracted from

  • Majtan, T.; Krijt, J.; Sokolova, J.; Krizkova, M.; Ralat, M.A.; Kent, J.; Gregory, J.F.; Kozich, V.; Kraus, J.P.
    Biogenesis of hydrogen sulfide and thioethers by cystathionine beta-synthase (2018), Antioxid. Redox Signal., 28, 311-323 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 S-adenosyl-L-methionine AdoMet is a V-type allosteric activator Homo sapiens

Application

EC Number Application Comment Organism
4.2.1.22 analysis development of a LC-MS/MS and HPLC methods to simultaneously measure formation of thioethers and hydrogen sulfide from mixtures of cystathionine beta-synthase substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 1
-
 L-homocysteine synthesis of L-cystathionine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.04
-
 L-homocysteine synthesis of L-cystathionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.27
-
 L-serine cosubstrate H2S, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.32
-
 L-serine cosubstrate H2S, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.41
-
 L-serine synthesis of L-cystathionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 2.13
-
 L-serine synthesis of L-cystathionine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 4.31
-
 L-homocysteine cosubstrate L-cysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 4.48
-
 L-homocysteine cosubstrate L-cysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 4.7
-
 H2S cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 5.02
-
 H2S cosubstrate L-serine, pH 7.4, 37°C Homo sapiens
4.2.1.22 8.11
-
 L-cysteine cosubstrate H2O, pH 7.4, 37°C Homo sapiens
4.2.1.22 8.41
-
 L-cysteine cosubstrate H2O, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 13.41
-
 L-cysteine cosubstrate L-homocysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 15.92
-
 L-cysteine cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens P35520
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-cysteine
-
 Homo sapiens H2S + L-serine
-
 ?
4.2.1.22 L-cysteine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2S
-
 ?
4.2.1.22 L-cysteine + L-serine
-
 Homo sapiens lanthionine + H2O
-
 ?
4.2.1.22 L-serine + H2S
-
 Homo sapiens L-cysteine
-
 ?
4.2.1.22 additional information cysteine is able to partially compete with serine on CBS, thus leading to generation of appreciable amounts of H2S. The leading H2S-producing reaction is condensation of cysteine with homocysteine, while cysteine desulfuration plays a dominant role when cysteine is more abundant than serine and homocysteine is limited. The serine-to-cysteine ratio is the main determinant of CBS H2S productivity. Abundance of cysteine over serine, for example, in blood plasma, allows for up to 43% of CBS activity being responsible for H2S production, while excess of serine typical for intracellular levels effectively limited such activity to less than 1.5%. CBS also produces lanthionine from serine and cysteine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 CBS
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.22 0.35
-
 H2S cosubstrate L-serine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.39
-
 L-serine cosubstrate H2S, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.62
-
 L-serine cosubstrate H2S, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.78
-
 H2S cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.06
-
 L-cysteine cosubstrate H2O, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.41
-
 L-cysteine cosubstrate H2O, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 3.67
-
 L-serine cosubstrate L-homocysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 4.66
-
 L-homocysteine cosubstrate L-serine, pH 7.4, 37°C Homo sapiens
4.2.1.22 7.09
-
 L-cysteine cosubstrate L-homocysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 8.17
-
 L-homocysteine cosubstrate L-cysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 12.7
-
 L-homocysteine cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 14.01
-
 L-serine cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 26.78
-
 L-homocysteine cosubstrate L-cysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 31.34
-
 L-cysteine cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.22 0.07
-
 H2S cosubstrate L-serine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.13
-
 L-cysteine cosubstrate H2O, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.17
-
 H2S cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.18
-
 L-cysteine cosubstrate H2O, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.28
-
 L-serine cosubstrate H2S, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.47
-
 L-serine cosubstrate H2S, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 0.53
-
 L-cysteine cosubstrate L-homocysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.93
-
 L-homocysteine cosubstrate L-cysteine, pH 7.4, 37°C Homo sapiens
4.2.1.22 1.98
-
 L-cysteine cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 2.63
-
 L-serine synthesis of L-cystathionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 4.61
-
 L-homocysteine synthesis of L-cystathionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 6.02
-
 L-homocysteine cosubstrate L-cysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 7.61
-
 L-serine synthesis of L-cystathionine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens
4.2.1.22 12.74
-
 L-homocysteine synthesis of L-cystathionine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C Homo sapiens