Any feedback?
Literature summary extracted from
- A continuous assay for L-talarate/galactarate dehydratase using circular dichroism (2018), Anal. Biochem., 544, 80-86 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.2.1.42 | analysis | a continuous assay for L-talarate/galactarate dehydratase is develped using circular dichroism. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.42 | analysis | a convenient, direct continuous circular dichroism-based assay is developed for following the L-talarate/galactarate dehydratase-catalyzed conversion of meso-galactarate to 5-dehydro-4-deoxy-D-glucarate. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.42 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.42 | expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | gene STM3697, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.42 | Tartronate | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 4.2.1.156 | additional information | tartronate is a much weaker inhibitor of enzyme StTGD than of mandelate racemase | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.156 | Tartronate | competitive inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.42 | 0.38 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.42 | 0.38 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.156 | additional information | - |
additional information | Michaelis-Menten kinetics | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.156 | L-talarate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.156 | L-talarate | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | - |
5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.156 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | ? | - |
? | |
| 4.2.1.156 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.42 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZL58 | - |
- |
| 4.2.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZL58 | - |
- |
| 4.2.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | Q8ZL58 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.42 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 DE3 by ultracentrifugation, nickel affinity chromatography, and dialysis | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.42 | galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
| 4.2.1.42 | galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
| 4.2.1.42 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.42 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.42 | meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.42 | meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.156 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.156 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
| 4.2.1.156 | additional information | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 4.2.1.156 | additional information | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.42 | L-talarate/galactarate dehydratase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | L-talarate/galactarate dehydratase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | More | cf. EC 4.2.1.42 | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | STM3697 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | StTGD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | TGD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.42 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.42 | 4.8 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.42 | 4.8 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.42 | 8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.156 | 8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.42 | 10.7 | - |
Tartronate | pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.42 | 10.7 | - |
Tartronate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.156 | evolution | L-talarate/galactarate dehydratase (TGD) is a member of the mandelate racemase (MR) subgroup of the enolase superfamily of enzymes. Members of the MR subgroup share with other members of the enolase superfamily a common bidomain structure (i.e., (beta/alpha)-7beta-barrel domain and an alpha+beta capping domain) and a common partial reaction (i.e., the metal-assisted, Broensted base-catalyzed abstraction of the alpha-proton from a carboxylate substrate to form an enol(ate) intermediate) | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.42 | 13 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.42 | 13 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
