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Literature summary extracted from
- A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima (2016), Anal. Biochem., 501, 75-81 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.7 | 2-mercaptoethanol | 100% activity at 0.5 mM | Thermotoga maritima |  |
| 5.4.2.7 | alpha-D-glucose1,6-bisphosphate | - |
Thermotoga maritima | |
| 5.4.2.7 | dithiothreitol | 100% activity at 0.5 mM | Thermotoga maritima | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.7 | expressed in Escherichia coli BL21 (DE3) cells | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.7 | 0.75 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 60°C | Thermotoga maritima | |
| 5.4.2.7 | 1.2 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 90°C | Thermotoga maritima | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.7 | Mn2+ | required, 100% activity at 0.1 mM | Thermotoga maritima | |
| 5.4.2.7 | additional information | the presence of Ni2+, Co2+, Zn2+, and Cu2+ enhances enzyme activities, but not as much as Mn2+ | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.7 | alpha-D-ribose 1-phosphate | Thermotoga maritima | - |
alpha-D-ribose 5-phosphate | - |
r | |
| 5.4.2.7 | alpha-D-ribose 5-phosphate | Thermotoga maritima | - |
alpha-D-ribose 1-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.7 | Thermotoga maritima | Q9WY14 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.7 | IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.7 | 14 | - |
crude enzyme, at pH 7.5 and 90°C | Thermotoga maritima |
| 5.4.2.7 | 57.8 | - |
after 4.1fold purification, at pH 7.5 and 90°C | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.7 | alpha-D-ribose 1-phosphate | - |
Thermotoga maritima | alpha-D-ribose 5-phosphate | - |
r | |
| 5.4.2.7 | alpha-D-ribose 5-phosphate | - |
Thermotoga maritima | alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.7 | ? | x * 42000, SDS-PAGE | Thermotoga maritima |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.7 | alpha-D-ribose1,5-phosphomutase | - |
Thermotoga maritima |
| 5.4.2.7 | PPM | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.7 | 90 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.7 | 30 | 100 | about 30% activity at 30°C, about 40% activity at 40°C, about 50% activity at 50°C, about 60% activity at 60°C, about 70% activity at 70°C, about 80% activity at 80°C, 100% activity at 90°C, about 90% activity at 100°C | Thermotoga maritima |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.7 | 55.6 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 60°C | Thermotoga maritima | |
| 5.4.2.7 | 185 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 90°C | Thermotoga maritima | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.7 | 7.5 | - |
in 100 mM MOPS buffer | Thermotoga maritima |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.7 | 6.5 | 8 | about 40% activity at pH 6.5, more than 70% activity between pH 7.0 and 8.0 | Thermotoga maritima |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.7 | 74.1 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 60°C | Thermotoga maritima | |
| 5.4.2.7 | 154 | - |
alpha-D-ribose 5-phosphate | at pH 7.5 and 90°C | Thermotoga maritima | |
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