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Literature summary extracted from

  • Gonzalez, J.M.; Marti-Arbona, R.; Chen, J.C.; Unkefer, C.J.
    Structure of Methylobacterium extorquens malyl-CoA lyase CoA-substrate binding correlates with domain shift (2017), Acta Crystallogr. Sect. F, 73, 79-85 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.24 expressed in Escherichia coli Arctic Express (DE3) cells Methylorubrum extorquens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.24 sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 30%(w/v) PEG 400, 0.2 M MgCl2 Methylorubrum extorquens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.24 (2R)-4-malyl-CoA competitive inhibitor Methylorubrum extorquens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.24 Mg2+ dependent on Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.24 (2S)-4-malyl-CoA Methylorubrum extorquens
-
 acetyl-CoA + glyoxylate
-
 r
4.1.3.24 acetyl-CoA + glyoxylate Methylorubrum extorquens
-
 (2S)-4-malyl-CoA
-
 r
4.1.3.24 propionyl-CoA + glyoxylate Methylorubrum extorquens
-
 (2S)-4-(beta-methyl)-malyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.24 Methylorubrum extorquens C5B113
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.24 HisTrap column chromatography and Superdex 200 gel filtration Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.24 (2S)-4-malyl-CoA
-
 Methylorubrum extorquens acetyl-CoA + glyoxylate
-
 r
4.1.3.24 acetyl-CoA + glyoxylate
-
 Methylorubrum extorquens (2S)-4-malyl-CoA
-
 r
4.1.3.24 propionyl-CoA + glyoxylate
-
 Methylorubrum extorquens (2S)-4-(beta-methyl)-malyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.24 homohexamer 6 * 34000, calculated from amino acid sequence Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
4.1.3.24 MCL
-
 Methylorubrum extorquens