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Literature summary extracted from

  • Fujii, T.; Yamauchi, T.; Ishiyama, M.; Gogami, Y.; Oikawa, T.; Hata, Y.
    Crystallographic studies of aspartate racemase from Lactobacillus sakei NBRC 15893 (2015), Acta Crystallogr. Sect. F, 71, 1012-1016 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.1.1.13 food industry the enzyme from the lactic acid bacterium Lactobacillus sakei NBRC 15893 is considered to be involved in D-aspartate synthesis during the brewing process of Japanese sake at low temperatures Latilactobacillus sakei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.13 recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Latilactobacillus sakei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.13 purified enzyme, sitting drop vapour diffusion method with microseeding, mixing of 0.002 ml of 20 mg/ml protein in 50 mM MES, pH 6.5, and 4 mM dithiothreitol, with 0.002 ml of reservoir solution containing 25% v/v PEG MME 550, and 5% v/v 2-propanol, and 0.1 M sodium acetate, pH 4.8, equilibration againat 0.1 ml reservoir solution, 20°C, single rod-shaped crystals, X-ray diffraction structure determination and analysis at 2.55 A resolution, molecular replacement calculations are carried out using the coordinates of PhAspR, PDB ID 1jfl, as a starting model Latilactobacillus sakei

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.13 Latilactobacillus sakei
-
 isolated from kimoto
-
5.1.1.13 Latilactobacillus sakei NBRC 15893
-
 isolated from kimoto
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.13 recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Latilactobacillus sakei

Synonyms

EC Number Synonyms Comment Organism
5.1.1.13 LsAspR
-
 Latilactobacillus sakei

General Information

EC Number General Information Comment Organism
5.1.1.13 physiological function the enzyme from the lactic acid bacterium Lactobacillus sakei NBRC 15893 is considered to be involved in D-aspartate synthesis during the brewing process of Japanese sake at low temperatures Latilactobacillus sakei