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Literature summary extracted from

  • Ignea, C.; Athanasakoglou, A.; Andreadelli, A.; Apostolaki, M.; Iakovides, M.; Stephanou, E.G.; Makris, A.M.; Kampranis, S.C.
    Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast (2017), Sci. Rep., 7, 8855 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.60 synthesis coexpression of mutant F112L or mutant V296L and cytochrome Cyp76AK6 in yeast leads to overall improvements of 24- and 14fold for pisiferic acid and salviol, respectively, compared to wild-type Salvia pomifera
1.14.14.175 synthesis coexpression of mutant F112L or mutant V296L and cytochrome Cyp76AK6 in yeast leads to overall improvements of 24- and 14fold for pisiferic acid and salviol, respectively, compared to wild-type Salvia pomifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.60 expression Saccaromyces cerevisiae Salvia pomifera
1.14.14.175 expression Saccaromyces cerevisiae Salvia pomifera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.60 molecular modeling of structure Salvia pomifera
1.14.14.175 molecular modeling of structure Salvia pomifera

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.60 F112L mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190, or manoyl oxide Salvia pomifera
1.14.14.60 V296L mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190 Salvia pomifera
1.14.14.175 F112L mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene Salvia pomifera
1.14.14.175 V296L mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene Salvia pomifera

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.60 Salvia pomifera
-
-
-
1.14.14.175 Salvia pomifera
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.60 trichome glandular trichome Salvia pomifera
-

Synonyms

EC Number Synonyms Comment Organism
1.14.14.60 CYP76AH24
-
 Salvia pomifera
1.14.14.175 CYP76AH24
-
 Salvia pomifera