Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kucera, J.; Pakostova, E.; Lochman, J.; Janiczek, O.; Mandl, M.
    Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in c? (2016), Res. Microbiol., 167, 357-366 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.9.1 gene cyc2, the gene is encoded in the iron oxidation system-encoding rus operon comprising genes cyc1, cyc2, coxA, coxC and rus, quantitative real-time PCR gene expression analysis in strain CCM 4253 Acidithiobacillus ferrooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.9.1 outer membrane
-
 Acidithiobacillus ferrooxidans 19867
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.16.9.1 Fe(II) + rusticyanin Acidithiobacillus ferrooxidans
-
 Fe(III) + reduced rusticyanin
-
 ?
1.16.9.1 Fe(II) + rusticyanin Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270
-
 Fe(III) + reduced rusticyanin
-
 ?
1.16.9.1 additional information Acidithiobacillus ferrooxidans direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 ?
-
 ?
1.16.9.1 additional information Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.16.9.1 Acidithiobacillus ferrooxidans O33823 strain CCM 4253 shows 100% identity to type strain ATCC 23270
-
1.16.9.1 Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 O33823 strain CCM 4253 shows 100% identity to type strain ATCC 23270
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.9.1 cell culture cells grown anaerobically on S0 as an electron donor and Fe3+ as an electron acceptor, gene transcript profile of gene cyc2 monitored throughout the cells' growth phases. Reduction in the abundance of Cyc2 in anaerobic cells Acidithiobacillus ferrooxidans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.9.1 Fe(II) + rusticyanin
-
 Acidithiobacillus ferrooxidans Fe(III) + reduced rusticyanin
-
 ?
1.16.9.1 Fe(II) + rusticyanin
-
 Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 Fe(III) + reduced rusticyanin
-
 ?
1.16.9.1 additional information direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 Acidithiobacillus ferrooxidans ?
-
 ?
1.16.9.1 additional information direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.16.9.1 Cyc2
-
 Acidithiobacillus ferrooxidans
1.16.9.1 outer-membrane iron oxidase
-
 Acidithiobacillus ferrooxidans
1.16.9.1 terminal Fe3+ reductase
-
 Acidithiobacillus ferrooxidans
1.16.9.1 terminal ferric iron reductase
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.9.1 cytochrome c
-
 Acidithiobacillus ferrooxidans
1.16.9.1 rusticyanin Rus, need for rusticyanin in the anaerobic electron transport pathway Acidithiobacillus ferrooxidans

General Information

EC Number General Information Comment Organism
1.16.9.1 malfunction cells lacking the Fe3+-reducing capacity reveal downregulation of energy metabolism proteins, which are in some cases even absent. Among the repressed and missing proteins are Cyc2, rusticyanin, heterodisulfide reductase (Hdr), thiosulfate:quinone oxidoreductase (Tqo) and sulfide:quinone reductase (Sqr) Acidithiobacillus ferrooxidans
1.16.9.1 metabolism the enzyme is involved in the involved in the anaerobic pathway of S0 oxidation coupled with dissimilatory Fe3+ reduction. The pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase), periplasmic electron shuttle rusticyanin, c4-type cytochrome CycA1, the inner membrane cytochrome bc1 complex I, and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. An alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Strain- or phenotype-dependent pathway variation, overview. The enzyme is involved in the anaerobic respiratory pathway, regulation of the pathway and model overview Acidithiobacillus ferrooxidans
1.16.9.1 physiological function ability of Acidithiobacillus ferrooxidans to anaerobically reduce Fe3+, molecular mechanism, overview. Rusticyanin is a stable and highly abundant protein in Fe2+-grown cells, in which it represents about 5% of soluble protein, that fulfills the role of an electron transporter in the respiratory chain, even after strong reduction of its content in a cell. The terminal Fe3+ reductase might be outer membrane cytochrome Cyc2, operating in reverse mode and reducing Fe3+ to Fe2+ under anaerobic conditions Acidithiobacillus ferrooxidans