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Literature summary extracted from

  • Vetting, M.; Bareich, D.; Yu, M.; Blanchard, J.
    Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nalpha-acetylation of ribosomal protein S18 (2008), Protein Sci., 17, 1781-1790 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.266 expressed in Escherichia coli Rosetta2 (DE3) pLysS cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.266 in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.266 CoA-S-acetyl-ARYFRR linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6) Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.266 acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome] Salmonella enterica subsp. enterica serovar Typhimurium
-
 CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.266 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZJW4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.266 Ni HiTrap column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.266 acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.266 RimI
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.266 0.0041
-
 CoA-S-acetyl-ARYFRR at pH 7.3 and 25°C Salmonella enterica subsp. enterica serovar Typhimurium