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Literature summary extracted from

  • Tuntland, M.L.; Wolf, N.M.; Fung, L.W.
    Differences in the purification and solution properties of PurC gene products from Streptococcus pneumoniae and Bacillus anthracis (2015), Protein Expr. Purif., 114, 143-148 .
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.6 drug development 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen Streptococcus pneumoniae
6.3.2.6 drug development 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.6 gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Streptococcus pneumoniae
6.3.2.6 gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.6 Mg2+ required Streptococcus pneumoniae
6.3.2.6 Mg2+ required Bacillus anthracis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.6 55000
-
 recombinant enzyme, gel filtration Bacillus anthracis
6.3.2.6 56000
-
 recombinant enzyme, gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae
-
 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Bacillus anthracis
-
 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae ATCC BAA-334 / TIGR4
-
 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.6 Bacillus anthracis Q81ZH5
-
-
6.3.2.6 Streptococcus pneumoniae Q07296
-
-
6.3.2.6 Streptococcus pneumoniae ATCC BAA-334 / TIGR4 Q07296
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration Streptococcus pneumoniae
6.3.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
 Streptococcus pneumoniae ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
 Bacillus anthracis ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
 Streptococcus pneumoniae ATCC BAA-334 / TIGR4 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.6 dimer 2 * 29166, sequence calculation, 2 * 29035, mass spectrometry Streptococcus pneumoniae
6.3.2.6 dimer 2 * 29701, sequence calculation, 2 * 29569, mass spectrometry Bacillus anthracis
6.3.2.6 More analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2 Streptococcus pneumoniae
6.3.2.6 More modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2 Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
6.3.2.6 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase
-
 Streptococcus pneumoniae
6.3.2.6 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase
-
 Bacillus anthracis
6.3.2.6 BaPurC
-
 Bacillus anthracis
6.3.2.6 PurC
-
 Streptococcus pneumoniae
6.3.2.6 PurC
-
 Bacillus anthracis
6.3.2.6 spPurC
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.6 25
-
 assay at Streptococcus pneumoniae
6.3.2.6 25
-
 assay at Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.6 8
-
 assay at Streptococcus pneumoniae
6.3.2.6 8
-
 assay at Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.6 ATP
-
 Streptococcus pneumoniae
6.3.2.6 ATP
-
 Bacillus anthracis

General Information

EC Number General Information Comment Organism
6.3.2.6 metabolism 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria Streptococcus pneumoniae
6.3.2.6 metabolism 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria Bacillus anthracis
6.3.2.6 additional information analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview Streptococcus pneumoniae
6.3.2.6 additional information modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview Bacillus anthracis