EC Number | Application | Comment | Organism |
---|---|---|---|
6.3.2.6 | drug development | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen | Streptococcus pneumoniae |
6.3.2.6 | drug development | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen | Bacillus anthracis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.6 | gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL | Streptococcus pneumoniae |
6.3.2.6 | gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL | Bacillus anthracis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.6 | Mg2+ | required | Streptococcus pneumoniae | |
6.3.2.6 | Mg2+ | required | Bacillus anthracis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.2.6 | 55000 | - |
recombinant enzyme, gel filtration | Bacillus anthracis |
6.3.2.6 | 56000 | - |
recombinant enzyme, gel filtration | Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Streptococcus pneumoniae | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Bacillus anthracis | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Streptococcus pneumoniae ATCC BAA-334 / TIGR4 | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.6 | Bacillus anthracis | Q81ZH5 | - |
- |
6.3.2.6 | Streptococcus pneumoniae | Q07296 | - |
- |
6.3.2.6 | Streptococcus pneumoniae ATCC BAA-334 / TIGR4 | Q07296 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration | Streptococcus pneumoniae |
6.3.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration | Bacillus anthracis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Streptococcus pneumoniae | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Bacillus anthracis | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
6.3.2.6 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Streptococcus pneumoniae ATCC BAA-334 / TIGR4 | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.2.6 | dimer | 2 * 29166, sequence calculation, 2 * 29035, mass spectrometry | Streptococcus pneumoniae |
6.3.2.6 | dimer | 2 * 29701, sequence calculation, 2 * 29569, mass spectrometry | Bacillus anthracis |
6.3.2.6 | More | analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2 | Streptococcus pneumoniae |
6.3.2.6 | More | modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2 | Bacillus anthracis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.2.6 | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase | - |
Streptococcus pneumoniae |
6.3.2.6 | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase | - |
Bacillus anthracis |
6.3.2.6 | BaPurC | - |
Bacillus anthracis |
6.3.2.6 | PurC | - |
Streptococcus pneumoniae |
6.3.2.6 | PurC | - |
Bacillus anthracis |
6.3.2.6 | spPurC | - |
Streptococcus pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.6 | 25 | - |
assay at | Streptococcus pneumoniae |
6.3.2.6 | 25 | - |
assay at | Bacillus anthracis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.6 | 8 | - |
assay at | Streptococcus pneumoniae |
6.3.2.6 | 8 | - |
assay at | Bacillus anthracis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.6 | ATP | - |
Streptococcus pneumoniae | |
6.3.2.6 | ATP | - |
Bacillus anthracis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.3.2.6 | metabolism | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria | Streptococcus pneumoniae |
6.3.2.6 | metabolism | 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria | Bacillus anthracis |
6.3.2.6 | additional information | analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview | Streptococcus pneumoniae |
6.3.2.6 | additional information | modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview | Bacillus anthracis |