Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ke, X.; Ding, G.; Ma, B.; Liu, Z.; Zhang, J.; Zheng, Y.
    Characterization of a novel CYP51 from Rhodococcus triatomae and its NADH-ferredoxin reductase-coupled application in lanosterol 14alpha-demethylation (2017), Process Biochem., 62, 59-68 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.36 expression in Escherichia coli Rhodococcus triatomae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.36 homoology modeling of structure Rhodococcus triatomae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.3 0.0344
-
 NADH pH and temperature not specified in the publication Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH Acinetobacter sp.
-
 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH Nocardia farcinica
-
 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.36 Rhodococcus triatomae M2UZT3
-
-
1.14.15.36 Rhodococcus triatomae BKS 15-14 M2UZT3
-
-
1.18.1.3 Acinetobacter sp.
-
-
-
1.18.1.3 Nocardia farcinica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.36 hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.36 hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae BKS 15-14 oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.36 lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.36 lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae BKS 15-14 hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.36 lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
 Rhodococcus triatomae 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.15.36 lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
 Rhodococcus triatomae BKS 15-14 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.15.36 additional information exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor Rhodococcus triatomae ?
-
 ?
1.14.15.36 additional information exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor Rhodococcus triatomae BKS 15-14 ?
-
 ?
1.14.15.36 oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae 4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.36 oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus triatomae BKS 15-14 4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH
-
 Acinetobacter sp. reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH
-
 Nocardia farcinica reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.36 ? x * 54000, SDS-PAGE Rhodococcus triatomae

Synonyms

EC Number Synonyms Comment Organism
1.14.15.36 CYP51
-
 Rhodococcus triatomae
1.14.15.36 G419_21379
-
 Rhodococcus triatomae
1.18.1.3 FdR
-
 Acinetobacter sp.
1.18.1.3 FdR
-
 Nocardia farcinica
1.18.1.3 Fdx-FdR
-
 Acinetobacter sp.
1.18.1.3 Fdx-FdR
-
 Nocardia farcinica
1.18.1.3 NADH-ferredoxin reductase
-
 Acinetobacter sp.
1.18.1.3 NADH-ferredoxin reductase
-
 Nocardia farcinica

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.3 NADH
-
 Acinetobacter sp.
1.18.1.3 NADH
-
 Nocardia farcinica