EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.18.1 | recombinant expression of codon-optimized CsPPO in Escherichia coli strain BL21(DE3), ectopic expression leads to the formation of inclusion bodies | Camellia sinensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | additional information | - |
additional information | enzyme kinetic analysis at different pH values, overview | Camellia sinensis | |
1.14.18.1 | 0.314 | - |
epicatechin | pH and temperature not specified in the publication | Camellia sinensis | |
1.14.18.1 | 0.479 | - |
catechin | pH and temperature not specified in the publication | Camellia sinensis | |
1.14.18.1 | 3.1 | - |
catechol | pH and temperature not specified in the publication | Camellia sinensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | Cu2+ | a copper-containing enzyme, copper content of 0.880 atom/molecule of protein in recombinant refolded enzyme | Camellia sinensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 catechol + O2 | Camellia sinensis | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
1.14.18.1 | catechin + O2 | Camellia sinensis | - |
? + 2 H2O | - |
? | |
1.14.18.1 | epicatechin + O2 | Camellia sinensis | - |
? + 2 H2O | - |
? | |
1.14.18.1 | additional information | Camellia sinensis | polyphenol oxidases (PPOs) are nuclear-encoded copper-containing metalloproteins involved in either the hydroxylation of monophenols to o-diphenols (EC 1.14.18.1, monophenol monoxinase, tyrosinase, and cresolase) or dehydrogenation of o-diphenols to o-quinones (EC1.10.3.1, diphenol oxygen oxidoreductase and catecholase). The enzyme from Camellia sinensis oxidizes epicatechins to yield theaflavins and thearubigins | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Camellia sinensis | C0L3S4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | recombinant refolded enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Camellia sinensis |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.14.18.1 | recombinant enzyme from inclusion bodies, extensive standardization of buffers and methods of refolding such as 1. dialysis, 2. on-column refolding, and 3. rapid dilution yield active PPO from solubilized inclusion bodies with copper content of 0.880 atom/molecule of protein, method development, detailed overview | Camellia sinensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | leaf | - |
Camellia sinensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 catechol + O2 | - |
Camellia sinensis | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.14.18.1 | catechin + O2 | - |
Camellia sinensis | ? + 2 H2O | - |
? | |
1.14.18.1 | epicatechin + O2 | - |
Camellia sinensis | ? + 2 H2O | - |
? | |
1.14.18.1 | additional information | polyphenol oxidases (PPOs) are nuclear-encoded copper-containing metalloproteins involved in either the hydroxylation of monophenols to o-diphenols (EC 1.14.18.1, monophenol monoxinase, tyrosinase, and cresolase) or dehydrogenation of o-diphenols to o-quinones (EC1.10.3.1, diphenol oxygen oxidoreductase and catecholase). The enzyme from Camellia sinensis oxidizes epicatechins to yield theaflavins and thearubigins | Camellia sinensis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | CsPPO | - |
Camellia sinensis |
1.14.18.1 | polyphenol oxidase | - |
Camellia sinensis |
1.14.18.1 | PPO | - |
Camellia sinensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 5 | - |
recombinant refolded enzyme | Camellia sinensis |