EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.25 | - |
Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | - |
Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.15.25 | 39000 | - |
gel fitlration, reductase component CymA | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | 39000 | - |
gel fitlration | Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.25 | Pseudomonas chlororaphis subsp. aureofaciens | P95461 and O84920 and O84919 | P95461 i.e. reductase subunit CymA, cf. EC 1.8.1.3, O84920 i.e. subunit CymB, O84919 i.e. subunit CymM | - |
1.18.1.3 | Pseudomonas chlororaphis subsp. aureofaciens | P95461 | enzyme acts as reductase subunit of p-cymene mono-oxygenase, EC 1.14.15.25 | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.15.25 | 70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | 70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month | Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.15.25 | ? | x * 38433, calculated, x * 38000, SDS-PAGE of reductase component CymA | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | ? | x * 38433, calculated, x * 38000, SDS-PAGE | Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.25 | 30 | - |
10 min, 42% loss of activity, NADH-cytochrome c reductase activity of subunit CymA | Pseudomonas chlororaphis subsp. aureofaciens |
1.14.15.25 | 50 | - |
10 min, 98% loss of activity, NADH-cytochrome c reductase activity of subunit CymA | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | 30 | - |
10 min, 42% loss of activity | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | 50 | - |
10 min, 98% loss of activity | Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.25 | 8.4 | - |
NADH-cytochrome c reductase activity of subunit CymA | Pseudomonas chlororaphis subsp. aureofaciens |
1.18.1.3 | 8.4 | - |
- |
Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.3 | FAD | 1.07 mol/mol of enzyme | Pseudomonas chlororaphis subsp. aureofaciens | |
1.18.1.3 | additional information | no activity with NADPH | Pseudomonas chlororaphis subsp. aureofaciens | |
1.18.1.3 | NADH | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
1.18.1.3 | [2Fe-2S]-center | presence of one [2Fe-2S] center per enzyme molecule | Pseudomonas chlororaphis subsp. aureofaciens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.15.25 | Pseudomonas chlororaphis subsp. aureofaciens | expression of subunits CymABM is induced by growth on p-cymene | up |
1.18.1.3 | Pseudomonas chlororaphis subsp. aureofaciens | expression of subunits CymABM is induced by growth on p-cymene | up |