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Literature summary extracted from

  • Dutta, T.; Dutta, A.; Chakraborty, J.; Sarkar, J.; Pal Chowdhury, P.; Gunsalus, I.
    Purification and properties of reductase of the three-component p-cymene methyl hydroxylase from Pseudomonas chlororaphis subsp. aureofaciens (2012), Process Biochem., 47, 1263-1267 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.25
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
-
Pseudomonas chlororaphis subsp. aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.25 39000
-
gel fitlration, reductase component CymA Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 39000
-
gel fitlration Pseudomonas chlororaphis subsp. aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.25 Pseudomonas chlororaphis subsp. aureofaciens P95461 and O84920 and O84919 P95461 i.e. reductase subunit CymA, cf. EC 1.8.1.3, O84920 i.e. subunit CymB, O84919 i.e. subunit CymM
-
1.18.1.3 Pseudomonas chlororaphis subsp. aureofaciens P95461 enzyme acts as reductase subunit of p-cymene mono-oxygenase, EC 1.14.15.25
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.25 70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month Pseudomonas chlororaphis subsp. aureofaciens

Subunits

EC Number Subunits Comment Organism
1.14.15.25 ? x * 38433, calculated, x * 38000, SDS-PAGE of reductase component CymA Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 ? x * 38433, calculated, x * 38000, SDS-PAGE Pseudomonas chlororaphis subsp. aureofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.25 30
-
10 min, 42% loss of activity, NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens
1.14.15.25 50
-
10 min, 98% loss of activity, NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 30
-
10 min, 42% loss of activity Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 50
-
10 min, 98% loss of activity Pseudomonas chlororaphis subsp. aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.25 8.4
-
NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 8.4
-
-
Pseudomonas chlororaphis subsp. aureofaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.3 FAD 1.07 mol/mol of enzyme Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 additional information no activity with NADPH Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 NADH
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3 [2Fe-2S]-center presence of one [2Fe-2S] center per enzyme molecule Pseudomonas chlororaphis subsp. aureofaciens

Expression

EC Number Organism Comment Expression
1.14.15.25 Pseudomonas chlororaphis subsp. aureofaciens expression of subunits CymABM is induced by growth on p-cymene up
1.18.1.3 Pseudomonas chlororaphis subsp. aureofaciens expression of subunits CymABM is induced by growth on p-cymene up