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Literature summary extracted from

  • Skinner, S.; Liu, W.; Hiruma, Y.; Timmer, M.; Blok, A.; Hass, M.; Ubbink, M.
    Delicate conformational balance of the redox enzyme cytochrome P450cam (2015), Proc. Natl. Acad. Sci. USA, 112, 9022-9027 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 E195C/A199C/C334A site-directed mutagenesis, substrate and cofactor binding of the mutant compared to the wild-type, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida
1.14.15.1 P450cam
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.1 25
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida
1.14.15.1 putidaredoxin cofactor putidaredxin-bound enzyme structure analysis using crystal structure, PDB ID 4JWS Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.15.1 additional information cofactor putidaredxin-bound and substrate-bound wild-type and E195C/A199C mutant enzymes, camphor-free, cyanide-bound P450cam, and apoenzyme structure analysis, structure analysis using crystal structure, PDB IDs 4JWS and 3L63. Cyanide and camphor can bind simultaneously in the active site Pseudomonas putida