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Literature summary extracted from

  • Lee, S.; Badieyan, S.; Bevan, D.R.; Herde, M.; Gatz, C.; Tholl, D.
    Herbivore-induced and floral homoterpene volatiles are biosynthesized by a single P450 enzyme (CYP82G1) in Arabidopsis (2010), Proc. Natl. Acad. Sci. USA, 107, 21205-21210 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.59 expression in Saccharomyces cerevisiae Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.58 modeling and substrate docking support an oxidative bond cleavage of the alcohol substrate via syn-elimination of the polar head, together with an allylic C-5 hydrogen atom Arabidopsis thaliana
1.14.14.59 modeling and substrate docking support an oxidative bond cleavage of the alcohol substrate via syn-elimination of the polar head, together with an allylic C-5 hydrogen atom Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.58 0.0027
-
(6E,10E)-geranyllinalool pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.14.14.59 0.0018
-
(3S,6E)-nerolidol pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.58 Arabidopsis thaliana Q9LSF8 enzyme catalyzes reactions of EC 1.14.14.58 and of EC 1.14.14.59
-
1.14.14.59 Arabidopsis thaliana Q9LSF8 enzyme catalyzes reactions of EC 1.14.14.58 and of EC 1.14.14.59
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.58 leaf highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves Arabidopsis thaliana
-
1.14.14.58 stem constitutively expressed Arabidopsis thaliana
-
1.14.14.59 leaf highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves Arabidopsis thaliana
-
1.14.14.59 stem constitutively expressed Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.58 (6E,10E)-geranyllinalool + [reduced NADPH-hemoprotein reductase] + O2
-
Arabidopsis thaliana (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
?
1.14.14.59 (3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2
-
Arabidopsis thaliana (3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.58 At3g25180
-
Arabidopsis thaliana
1.14.14.58 CYP82G1
-
Arabidopsis thaliana
1.14.14.59 At3g25180
-
Arabidopsis thaliana
1.14.14.59 CYP82G1
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.58 0.11
-
(6E,10E)-geranyllinalool pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.14.14.59 0.2
-
(3S,6E)-nerolidol pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.58 cytochrome P450
-
Arabidopsis thaliana
1.14.14.59 cytochrome P450
-
Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.58 50
-
(6E,10E)-geranyllinalool pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.14.14.59 110
-
(3S,6E)-nerolidol pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana