EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.81 | YL-1 subunit of MPEC by map-based cloning, quantitative real-time PCR expression analysis, phylogenetic analysis | Oryza sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.81 | additional information | generation of subunit YL-1 mutants yl-1, phenotype, overview. The yl-1 mutant shows a yellow leaf and panicle phenotype with reduced chlorophyll accumulation through the whole growth phases. Mutant yl-1 exhibits the temperature-independent yellow leaf phenotype, it presents abnormal chloroplast development and attenuated photosynthetic efficiency | Oryza sativa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.81 | chloroplast | - |
Oryza sativa | 9507 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.81 | magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | Oryza sativa | - |
3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.81 | Oryza sativa | - |
cv. Zhongjiazao 17, grown in paddy fields during normal growing seasons at the China National Rice Research Institute, in Hangzhou, as well as in a growth chamber | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.81 | leaf | - |
Oryza sativa | - |
1.14.13.81 | additional information | enzyme subunit YL-1 protein localizes in chloroplasts, and is mainly expressed in green tissues, with greatest abundance in leaves and young panicles | Oryza sativa | - |
1.14.13.81 | panicle | - |
Oryza sativa | - |
1.14.13.81 | seedling | - |
Oryza sativa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.81 | magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | - |
Oryza sativa | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.81 | ? | x * 47000, recombinant enzyme subunit YL-1, SDS-PAGE | Oryza sativa |
1.14.13.81 | More | YL-1 encodes a subunit of MPEC | Oryza sativa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.81 | magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Oryza sativa |
1.14.13.81 | MPEC | - |
Oryza sativa |
1.14.13.81 | MPME cyclase | - |
Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.81 | NADPH | - |
Oryza sativa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.81 | evolution | phylogeny of the YL-1 protein family, overview | Oryza sativa |
1.14.13.81 | malfunction | chlorophyll biosynthesis upstream genes are highly expressed in the yl-1 mutant, while downstream genes are compromised, indicating that enzyme MPEC plays a pivotal role in the chlorophyll biosynthesis. The yl-1 mutant shows a yellow leaf and panicle phenotype with reduced chlorophyll accumulation through the whole growth phases. Mutant yl-1 exhibits the temperature-independent yellow leaf phenotype, it presents abnormal chloroplast development and attenuated photosynthetic efficiency. The mutant yl-1 affects mRNA accumulation levels of Chl biosynthesis process | Oryza sativa |
1.14.13.81 | physiological function | an essential enzyme during chlorophyll (Chl) biosynthesis. The enzyme subunit protein YL-1 is required for plastid membrane stability. The formation of the isocyclic ring is an aerobic reaction catalyzed by MPEC in chloroplasts, which controls the conversion of magnesium-protoporphyrin IX 13-monomethyl ester to divinyl protochlorophyllide (DVpchlide). The function of YL-1 in MPEC complex is essential for normal chlorophyll biosynthesis | Oryza sativa |