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Literature summary extracted from

  • Matsuba, Y.; Zi, J.; Jones, A.D.; Peters, R.J.; Pichersky, E.
    Biosynthesis of the diterpenoid lycosantalonol via nerylneryl diphosphate in Solanum lycopersicum (2015), PLoS ONE, 10, e0119302 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.142 expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.142 0.0038
-
 isopentenyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum
2.5.1.142 0.0229
-
 Z,Z-farnesyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.142 dimethylallyl diphosphate + 3 isopentenyl diphosphate Solanum lycopersicum
-
 3 diphosphate + nerylneryl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.142 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.142 HIS-Select HF nickel affinity Gel chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.142 leaf
-
 Solanum lycopersicum
-
2.5.1.142 petiole
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.142 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Solanum lycopersicum 3 diphosphate + nerylneryl diphosphate
-
 ?
2.5.1.142 dimethylallyl diphosphate + Z,Z-farnesyl diphosphate
-
 Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.142 CPT2
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.142 0.0026
-
 isopentenyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum
2.5.1.142 0.0026
-
 Z,Z-farnesyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.142 8 8.5
-
 Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.142 0.11
-
 Z,Z-farnesyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum
2.5.1.142 0.68
-
 isopentenyl diphosphate at pH 7.5 and 30°C Solanum lycopersicum