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Literature summary extracted from

  • Eljounaidi, K.; Cankar, K.; Comino, C.; Moglia, A.; Hehn, A.; Bourgaud, F.; Bouwmeester, H.; Menin, B.; Lanteri, S.; Beekwilder, J.
    Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway (2014), Plant Sci., 223, 59-68 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.150 gene CYP71Bl5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, transient expression in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain AGL0 electrotransformation leading to production of costunolide in tabacco leaves determined as costunolide-cysteine and costunolide-glutathione Cynara cardunculus var. altilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.150 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Cynara cardunculus var. altilis
-
 (+)-costunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.150 Cynara cardunculus var. altilis A0A059WMF6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.150 leaf
-
 Cynara cardunculus var. altilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.150 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
 Cynara cardunculus var. altilis (+)-costunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.150 CcaCOS
-
 Cynara cardunculus var. altilis
1.14.14.150 COS
-
 Cynara cardunculus var. altilis
1.14.14.150 CYP71Bl5
-
 Cynara cardunculus var. altilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.150 cytochrome P-450
-
 Cynara cardunculus var. altilis
1.14.14.150 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Cynara cardunculus var. altilis